摘要
目的研究麻黄Ephedra sinicaStapf.药材生物碱类成分的指纹图谱测定方法,确定杭锦旗产麻黄Ephedra sinicaStapf.药材HPLC标准指纹图谱。方法采用Lichrospher C18柱(4.6 mm×250 mm,5μm),乙腈-水-三乙胺-磷酸(5∶95∶0.05∶0.1)为流动相,流速:1mL/min。结果获得5个共有指纹峰。结论该方法准确、可靠,为更好地控制麻黄的内在质量提供了科学依据。
Objective To study the HPLC fingerprints of Ephedra sinica Stapf. and establish HPLC standard fingerprints chromatography from Hangjinqi planting bases. Methods Separation was performed on Lichrospher C18 （4.6 × 250 mm, 5μm） analytical column. Acetonitrile-water-TEA- phosphoric acid （ 5 ： 95 ： 0. 05 ： 0. 1）as mobile phase. The flow rate was 1 mL·min^-1. Results Fingerprints consisted of 5 common characteristic peaks. Conclusion This method was accurate, reliable and provides a scientific basis for control the quality of Ephedra sinica stapf.
出处
《现代中药研究与实践》
CAS
2006年第1期35-38,共4页
Research and Practice on Chinese Medicines
基金
国家十五科技攻关项目(2001BA701A41-1)
关键词
麻黄
HPLC
指纹图谱
Ephedra sinica Stapf.
HPLC
fingerprints
