摘要
等电点(IEP)是蛋白质最重要的物理化学性质之一,目前有多种测定等电点的方法。本文利用最简单的浊度/分光光度法和最先进的毛细管等电点聚焦(CaIPF)法分别测定了河南牛皮明胶的等电点,对两种方法进行比较,并论证了后一种方法的优势。
Isoelectric point(IEP) is one of the most important physicochemical properties of protein. There are many different methods for the determination of IEP. in this paper, a comparative study between the simplest turbidimetry/spectrometry method and the most advanced capillary IEP focusing(CaIPF) method was made. Henan cattle hide gelatin's IEP was determined with the two methods and the advantages of the CaIPF method over the other methods were demonstrated and discussed.
出处
《明胶科学与技术》
CAS
2006年第1期44-47,共4页
The Science and Technology of Gelatin