摘要
目的探讨蕈样肉芽肿(MF)不同时期浸润性皮损中增生性T细胞与树突细胞的分布特征。方法皮肤肿瘤组织石蜡切片,单克隆抗体免疫组化染色。结果 MF皮损中Ki-67+细胞和皮肤淋巴细胞相关抗原阳性(CLA+)细胞均明显增多,多数Ki-67+细胞CD4+和cLA+,MF肿瘤期真皮浸润组织中的 Ki-67+细胞明显多于斑片期／斑块期(P<0．01),且形态多不规则,异型性明显;未见CD83+／Ki-67+树突细胞,但树突细胞周围可见大量的Ki-67+增生性T细胞,CD83+树突细胞与Ki-67+肿瘤T细胞紧密接触。结论 CLA+／Ki-67+T细胞在MF皮损中表达的程度和免疫病理特征对于MF的早期发现和转化进展可能具有免疫病理学诊断意义。
Objective To evaluate the profile of proliferating T cells and dendritic cells （ DCs ） in the skin infiltrates of patients with mycosis fungnides （ MF ） in different stages. Method Paraffin section and immunohistochemisty with monoclonal antibody were used to detect the expression of special antigen per section. Results The numbers of Ki-67^＋ cells and cutaneous lymphocyte-associated antigen （ CLA^＋ ） cells both increased significantly in the skin infiltrates of MF. Most Ki-67^＋ cells expressed both CLA and CD4 antigen. The number of Ki-67^＋ cells was significantly higher （ P 〈 0.01 ） in the skin infiltrates in tumor stage than that in plaque stage, and most of the cells were irregular in shape with obvious heteromorphism. No CD83^＋/Ki-67^＋ DC was observed. However, the DCs were always surrounded by Ki-67^＋ cells, and CD83^＋ DCs were closely contacted with Ki-67^＋ cells. Conclusion The number and immunopathologic feature of CLA^＋/Ki-67^＋ cells in the skin infiltrates of MF may have important diagnostic value for the early diagnosis and evaluation of prognosis of MF.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2006年第4期207-209,共3页
Chinese Journal of Dermatology
