摘要
目的探讨p38α通路和血红素氧合酶(HO-1)在乳腺癌发生和耐药中的作用。方法应用MTT法和流式细胞术检测30例人乳腺癌细胞的增殖和凋亡变化,应用RT-PCR法检测p38α、 HO-1 mRNA表达。结果 78％的乳腺癌组织标本p38α mRNA表达水平显著高于正常组织的表达, 其中有淋巴结转移标本的表达水平高于无淋巴结转移者(P<0．01)。用吡喃阿霉素活化后在乳腺癌 MCF-7／ADR细胞中的表达高于MCF-7细胞。结论 p38α、HO-1在乳腺癌组织中高表达可能在人乳腺癌的过度增殖、凋亡受阻中起着重要作用;吡喃阿霉素经p38通路诱导乳腺癌细胞HO-1 mRNA 表达,可能与乳腺癌细胞的耐药机制有关。
Objective To investigate the rule of p38α pathway and HO-1 in the genesis and chemotherapy resistance of breast cancer. Methods The proliferation and apoptosis of human breast cancer cells were examined by MTT assay. The expression of p38α and HO-1 mRNA were examined by RT-PCR. Results The p38α mRNA level in 78% of samples was significantly greater than that in the normal tissue and the p38α mRNA level in patients with lymph node metastasis was higher than that without lymph node metastasis （ P 〈 0. 01 ）. The HO-1 activated with 5 μmol/L pirarubicin increased more in MCF-7/ADR cells than in MCF-7 cells, and was completely blocked by p38α inhibitor in MCF-7/ADR but only partially in the MCF-7 cell line. Conclusion HO-1 activation via p38α MAPK may play an important rule in the development of chemotherapy resistance.
出处
《中华普通外科杂志》
CSCD
北大核心
2006年第3期215-217,共3页
Chinese Journal of General Surgery
基金
中华医学会CMB基金ORF9807教育部留学回国启动基金F438
