摘要
对四溴荧光素(TBF)、四氯四溴荧光素(TTF)两种卤代荧光素滤纸基质室温磷光光谱(PS-RTP)特性及其与DNA的作用进行了研究。结果表明:TBF和TTF的PS-RTP的最大λex/λem为526/652nm和557/699nm;酸度实验表明:两种物质在碱性范围内有强发射。小牛胸腺(DNA)的存在会使TBF和TTF的PS-RTP强度发生变化;偏振实验表明:TBF、TTF与DNA的作用方式有嵌插作用;TBF和TTF的磷光寿命分别为136.4ms和131.0ms属长寿命磷光。
The phosphorescence characteristics of two halogen fluoresceins of tetrabromofluorescein（TBF） and tetrachlorotetrabromofluorescein（TTF） in paper substrate at room temperature and their interaction with DNA were studied. The maximal excitation and emission wavelengths were 526nm/652nm for TBF and 557nm/699nm for TTF,respectively,and they can emit strong RTP in basic medium with lifetime of 136.4ms and 131.0ms,respectively. The PS-RTP intensities of TBF and TTF are changed in the presence of DNA with TBF and TTF intercalations.
出处
《光谱实验室》
CAS
CSCD
2006年第2期295-299,共5页
Chinese Journal of Spectroscopy Laboratory
基金
国家自然科学基金(20575037)
山西省自然科学基金(20051012)资助项目
关键词
四溴荧光素
四氯四溴荧光素
DNA
滤纸基质室温磷光.
Tetrabromofluorescein, Tetrachlorotetrabromofluorescein, DNA, Paper Substrate Room Temperture Phosphorescence.
