摘要
目的:探讨楮实子红色素(Fructus B roussonetiaHaem atochrom e,FBH)体外抗氧化作用。方法:采用H2O2和Fe2+系统产生羟基自由基,NADH-PMS-NBT系统产生超氧阴离子自由基,并观察了FBH对羟基自由基及超氧阴离子自由基清除效果以及对H2O2诱导小鼠红细胞溶血的抑制和对肝组织匀浆自氧化终产物MDA及VC-Fe2+系统引起的线粒体的形态改变的抑制作用。结果:FBH能显著清除超氧阴离子及羟基自由基,抑制H2O2诱导小鼠红细胞溶血和肝匀浆自氧化,对肝线粒体也有保护作用。结论:FBH有较强的体外抗氧化作用。
Objective： To investigate the antioxidative effects of Fruetus Broussonetia Haematoehrome. Methods： N ADH-PMS- NBT system was used to produce supetoxide free radical （O2^-＋）, Fe^2＋-H2O2 system to generate hydroxyl free radical （ ·OH）, H2O2 to stimulate oxidative hemolysis of erythroeytes of mice. MDA production in liver homogenate induced by auto-oxidation was measured by MDA kits method, Results： FBH not only scavenged O^-＋and· OH produced by the experimental systems directly, but also inhibited H2O2 stimulated oxidative hemolysis of erythroeytes of mice, depressed MDA production in mice liver homogenate by auto-oxidation and hepatic mitochondria expanded induced by Vit C-Fe^2＋ system. Conclusion： FBH possess an antioxidative activity.
出处
《中药材》
CAS
CSCD
北大核心
2006年第3期262-265,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(No.30400595)
上海市联合利华研究与发展基金(03121)资助项目
