摘要
目的在同一反应体系中同时快速检测人子宫颈常见的人乳头状瘤病毒16型(HPV-16)、人疱疹病毒2型(HSV-2)和巨细胞病毒(CMV)以及沙眼衣原体(CT)感染。方法采用快速制备样品的方法简化了聚合酶链反应(PCR)实验过程,应用多重聚合酶链反应检测四种病原体。结果整个 PCR 检测过程仅需4小时,总检出率为57%,各病原体检出率分别是 HPV-16 11%、HSV-2 21%、CMV 14%和 CT21%。结论该方法既保持了 PCR 的敏感性和特异性,又是一种简便、快速的诊断技术,可用于人宫颈感染的临床诊断和预后判断。
Objective To simultaneously detect the common virus HPV-16,HSV-2,CMV and CT in human cervix by using a same PCR system.Methods The experiment was performed by PCR quick DNA sample preparation method and simple PCR processes,four pathogens were detected by mul- tiplex PCR.Results The whole process took only about four hours and the total dection rate was 57%,and HPV-16 11%,HSV-2 21%,CMV 14% and CT 21%,respectively.Conclusions This new PCR method is not only sensitive and specific,but also acts as a simple and rapid technique for diag- nosis.It may be applied to clinical diagnosis and evaluation of prognosis.
关键词
宫颈糜烂
乳头状瘤病毒
疱疹病毒
聚合酶链反应
Cervix erosion Papillomaviruses Herpesvirus hominis Cytomegaloviruses Chlamydia trachomatis Polymerase chain reaction
