摘要
本文研究了不同提取条件对豆豉提取物乙酰胆碱酯酶(AChE)抑制能力的影响,并且对9种市售豆豉体外AChE抑制能力进行了测定,用IC50表示豆豉对AChE抑制能力的大小。用不同溶剂(水、甲醇、乙醇)提取豆豉,发现乙醇提取物的IC50最小为:0.023mg/ml,水提取物没有AChE抑制能力。研究还发现用乙醇提取时,豆豉抑制AChE的IC50值随乙醇浓度,固液比值的减小而增大。不同提取时间的IC50值无显著性差异。9种豆豉80%乙醇提取液抑制AChE的IC50值范围为:0.033～1.110mg/ml。研究结果表明豆豉内含有抑制AChE的活性成分,有开发成为预防老年性痴呆症新型功能食品的潜质。
The effects of different extract conditions on acetylcholinesterase（AChE） inhibitory activities of Douchi were studied. The in vitro AChE inhibitory activities of 9 kinds of commercial Douchi were assayed, expessed as IC50. Ethanol extracts showed highest AChE inhibitory activity with IC50 value as 0.023mg/ml, while water extracts did not show any inhibition. When extracted by ethanol, the IC50 of Douchi inceased with the decrease of solid/liquid rate and ethanol concentration. No significant differences were observed within IC50 values of the Douchi extracts with different extract time. 80% ethanol extracts of these nine Douchi inhibited human acetylcholinesterase with IC50 values ranged from 0.033 to 1.110mg/ml. This novel finding suggests that Douchi containing anti-acetylcholinesterase components, might be used to retard the progression of Alzheimer disease, and indicates the potential to be developod into new functional foods.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第3期87-90,共4页
Food Science
基金
"中日国际合作项目"和"达能膳食营养研究与宣教基金"联合资助
