摘要
目的:选择杜仲叶醇提物中黄酮含量的测定方法。方法:分别采用紫外分光光度法、AlCl3显色法和Al(NO3)3络合法测定杜仲叶醇提液中黄酮的含量,并对测量结果进行了比较。结果:紫外分光光度法测得黄酮含量为4.4025mg/mL,数据偏高;络合法中,AlCl3显色法测定结果为0.2073mg/mL,低于Al(NO3)3络合法(0.4889mg/mL),甚至低于HPLC法测得的纯化后杜仲叶醇提液中黄酮含量(0.3201mg/mL)。结论:Al(NO3)3络合法测定结果较另外两种方法准确,是测定杜仲叶醇提液中总黄酮含量的有效方法。
Objective： To choose an appropriate method to measure the flavonoids in extracts from Evcommia Vlmolcles Oliv. leaves. Method： The content of flavonoids in the extracts from Evcommia Vlmoides Oliv. leaves were determined and compared by UV, AlCl3 complexing-spectrophotometry and Al（NO3）3 colorimetry. Results： The result by UV, 4.4025mg/mL, was the highest. The result by AAlCl3 eomplexing-spectrophotometry was 0.2073 mg/mL and was lower than that by Al（NO3）3 colorimetry, which is 0.4889 mg/mL, even lower than the content of flavonoids in the extracts more pured determined by HPLC. Conclusion： Al（NO3）3 complexing-spectrophotometry is an effective suitable method to measure the flavonoids in extracts from Evcommia Vlmoides Oliv. leaves.
出处
《中国食品学报》
EI
CAS
CSCD
2006年第1期224-229,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
江西省科技厅2004工业攻关项目
