摘要
目的检测KISS-1及GPR54mRNA在子宫内膜癌组织中的表达,探讨其在子宫内膜癌浸润及转移中的作用。方法采用逆转录多聚酶链反应(RT-PCR)法检测32例子宫内膜癌,10例子宫内膜上皮内瘤样病变(EIN)及12例正常子宫内膜组织中KiSS-1及GPR54mRNA的表达情况和其与各种临床参数的关系。结果KiSS-1mRNA在子宫内膜癌组中的表达率(37.5%)明显低于EIN组(80.0%)及正常内膜组(83.3%),并且其在子宫内膜癌组中的表达率与临床分期、肌层浸润深度及淋巴结转移密切相关,P均<0.05。GPR54mRNA在子宫内膜癌组的表达率(78.1%)高于EIN组(70.0%)和正常内膜组(66.7%),但无明显差异(均P>0.05)。GPR54mRNA在子宫内膜癌组中的表达与临床分期、组织分级、肌层浸润深度及淋巴结转移情况均无相关性(均P>0.05)。结论KiSS-1与其受体GPR54结合在抑制子宫内膜癌的浸润和转移过程中可能起着重要的作用。
[Objective] To probe the clinical significance of KISS-1 and GPR54 in endometrial carcinomas, we detected the expression of KISS-1 and GPR54 mRNA in endometrial carcinoma tissues. [Methods] The expression of KISS-1 and GPR54 mRNA in 32 patients with cndometrial carcinoma, 10 patients with endometrial intraepithelial neoplasia （EIN） and 12 patients with normal cndometrlum were examined by rcverse transeriptase polymerase chain reaction （RT-PCR） method. [Results] KISS-1 mRNA had a lower expression in patients with endometrial carcinoma （37.5%） than in patients with EIN （80.0%） and normal endometrium （83.3%）. The expression of KISS-1 mRNA in patients with endometrial carcinoma con＇elated with their clinical stage, myometrial invasion and lymph node metastasis （P 〈0.05）. GPR54 mRNA had a higher expression in patients with endometrial carcinoma （78.1%） than in patients with EIN （70.0%） and normal endometrium （66.7%）, but there was no difference among them （P 〉0.05）. The expression of GPR54 mRNA in patients with endometrial earcinoma had nothing to do with their clinical stage, histology grade, myometrial invasion and lymph node metastasis （P 〉0.05）. [Conclusions] The interaction of KISS-1 and GPR54 may play an important role in inhibiting the invasion and metastasis of endometrial carcinoma.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2006年第6期813-816,821,共5页
China Journal of Modern Medicine
