摘要
报道了通过毛细管电泳-安培检测法测定腺嘌呤(A)和鸟嘌呤(G)来间接测定酵母菌中核糖核酸含量的方法.探讨了电极电位、运行缓冲溶液、分离电压及进样时间等对毛细管电泳分离-安培检测的影响,得到了较为优化的测定条件.在30 mmol/L的硼酸盐缓冲液(pH 9.0)中,上述两组分可在18 min内完全分离.腺嘌呤和鸟嘌呤的检测限分别为2.0×10-7mol/L和1.7×10-7mol/L.该法具有较好的重现性和灵敏度,可以用于食品中酵母菌中核糖核酸的快速测定.
The indirect determination of the content of RNA in yeast by analyzing adenine （A） and guanine （G） with capillary electrophoresis with amperometric detection （CE-AD） was repor ted. The effects of potential of working electrode, running buffer, separation voltage and injection time on the CE-AD determination were investigated. Adenine and guanine could be well separated in a 30 mmol/L horate buffer （pH 9. 0） within 18 rain under the optimum conditions. Their detection limits of adenine （S/N=3） of 2.0×10^-7mol/L and 1.7×10^-7mol/L, respectively. The experimental results showed that this method is of high sensitivity and high repeatability, and potential to be used in the quick analysis of yeast in foods.
出处
《华东师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第2期7-12,共6页
Journal of East China Normal University(Natural Science)
基金
上海市科委自然科学基础研究基金(04ZR14041)
关键词
毛细管电泳
安培检测
酵母菌
核糖核酸
capillary electrophoresis
amperometric detection
yeast
ribonucleic acid （RNA）
