摘要
在附加不同浓度NaCl及2.4-D2mg/L的MS培养基上,甘蔗愈伤组织表现出不同的生长效应。培养基中NaCl浓度低时(1%以下),甘蔗愈伤组织表现为适应性生长;而在高NaCl浓度时(2%以上),愈伤组织生长表现出盐害特征。脯氨酸、还原糖测定结果显示,在盐适应情况下,甘蔗愈伤组织中的脯氨酸含量较高,还原糖含量较低;在盐害情况下则相反。
On MS basal medium supplemented with 2 mg/L 2.4-D and different concentrations of NaCl, sugarcane callus shows different growth effects. As he concentrations of NaCl in me- dium are low (<1%), the callus appears in adaptive growth and as the concentrations of NaCl are high (>2%), the callus is to be salt-injured. The levels of proline and reducing sugar of callus were measured and the results shows that the salt-adapted callus has a high level proline and low level reducing sugar, the salt-injured callus has a low level proline and high level reducing sugar.
出处
《广西科学院学报》
1992年第2期59-63,共5页
Journal of Guangxi Academy of Sciences
关键词
甘蔗
愈伤组织
脯氨酸
还原糖
盐
Sugarcane callus
NaCl
Proline
Reducing sugar