摘要
C.elegans fat-1基因来源于小秀丽线虫(Caenorhabditis elegans),翻译产物是n-3 PUFAs脱氢酶。n-3 PUFAs脱氢酶以n-6 PUFAs为底物进行脱氢反应,生成相应的n-3 PUFAs,改变细胞膜n-6/n-3 PUFAs的比例,优化来源于n-6 PUFAs的类花生酸的组成。体外实验表明,C.elegans fat-1 cDNA的表达可促进肿瘤细胞凋亡,抑制肿瘤细胞增殖,并可下调与肿瘤细胞黏附和转移相关基因的表达。此基因的抗肿瘤机制不甚明确,可能是通过改变细胞n-6/n-3 PUFAs的比例从而触发了下游的抗肿瘤机制。
Caenorhabditis Elegans fat-1 gene comes from Caenorhabditis Elegans and the translational product of the gene is n-3 PUFAs dehydrognase, which achieves dehydrogenation by using n-6 PUFAs as the substrate thereby balancing the ratio of n-6 to n-3 PUFAs and optimize the constitution of eicosanoid coming form n-6 PUFAs. In vitro studies showed that the expression of Caenorhabditis Elegans fat-1 cDNA can promote the apoptosis of tumor cells, inhibit the proliferation of tumor cells and down-regulate the expression of genes relating to the adhesion and metastasis of tumor ceils. The exact anti-tumor mechanisms of the gene remain unclear and it may work by changing the ratio of n-6 to n-3 PUFAs, thereby triggering the down-stream anti-tumor mechanisms.
出处
《医学分子生物学杂志》
CAS
CSCD
2006年第2期118-121,共4页
Journal of Medical Molecular Biology
基金
国家自然科学基金(No.30370467)~~
