摘要
AIM： To determine whether increased blood flow of the liver can cause oxidative stress and hepatocyte damage, and to elaborate methods suitable for measuring the antioxidant defence during hepatic surgery on rat model.METHODS： In nembutal narcosis, the left lateral and the medial lobes of the liver were clipped for 45 rain to make the total blood supply flow through the other lobes. Total antioxidant status, glutathione peroxidase and superoxide dysmutase activity, as well as the concentrations of diene conjugates and free sulphydril groups, H-donating ability and reducing power of the liver samples were determined. Chemiluminescent intensity of the liver was also measured. Metal ions （Al, Ca, Cu, Fe, Mg, Mn, Zn） and P and S concentrations of the liver were determined with an inductively coupled plasma optical emission spectrometer and Se content was measured by cathodic stripping voltammetry.RESULTS： Glutathione peroxidase and superoxide dysmutase activities of the liver decreased significantly in the hyperemia group compared to those observed in the sham operated group. The level of total antioxidant status was also significantly lower in the hyperemia group. H-donating ability, reducing power and free sulphydril group concentration showed the same tendency. A significant correlation （P〈0.05） was found between the changes in non-specific antioxidant activities. This pointed to simultaneous activity of the antioxidant defence system. Al, Cu, Mn, Zn, and S were lower in the hyperemia group than in the sham operated group when the levels of Ca, Fe, Mg, Se and P ions were higher during hyperemia.CONCLUSION： Oxidative stress is one of the main factors for the injury of intact liver lobes during ischaemia-reperfusion.
瞄准：为了决定是否增加了，并且详细描述对在老鼠模型上在肝的外科期间测量抗氧化剂防卫合适的方法，肝的血流能引起氧化应力和 hepatocyte 损坏。方法：在戊巴比妥钠昏迷状态，侧面的左和肝的中间的脑叶被抓紧让 45 min 让全部的血供给通过另外的脑叶流动。全部的抗氧化剂地位，谷胱甘肽过氧化物酶和超级氧化物 dysmutase 活动，以及 diene 的集中结合，免费 sulphydril 组织，捐赠 H 能力和肝样品的减少的力量被决定。肝的 Chemiluminescent 紧张也被测量。金属离子(艾尔， Ca， Cu， Fe， Mg， Mn， Zn ) 并且肝的 P 和 S 集中与诱导地联合的血浆被决定光排放分光计和 Se 内容被阴极的的脱衣的 voltammetry 测量。结果：谷胱甘肽过氧化物酶和肝的超级氧化物 dysmutase 活动与在假冒的操作的组观察的那些相比在充血组显著地减少了。全部的抗氧化剂地位的水平在充血组也是显著地更低的。捐赠 H 能力，减少的力量和免费 sulphydril 组集中显示出一样的趋势。重要关联(P<0.05 ) 在非特定的抗氧化剂活动在变化之间被发现。这指向了抗氧化剂防卫系统的同时的活动。当 Ca， Fe， Mg， Se 和 P 离子的层次在充血期间是更高的时，艾尔， Cu， Mn， Zn，和 S 比在假冒的操作的组在充血组是更低的。结论：氧化应力在 ischaemia-reperfusion 期间是为未经触动的肝脑叶的损害的主要因素之一。
基金
Supported by the Ph.D.Programme of the Semmelweis University No 2/1,ETT 002/2003 Project
1A 005/2004,1B 047/2004 NKFP Projects
