摘要
目的建立一种可以用于免疫分析的微流控芯片系统。方法采用激光直接加工法制备微流控芯片，建立由流体拉制系统、免疫反应系统和信号处理系统构成的芯片系统，用于甲胎蛋白的测定。结果芯片系统可以在20min内完成AFP的分析，所需要的标本量和试荆量为5μl，线性范围为1-800ng／ml，批内CV平均为4．8％，批间CV平均为7．0％，回收率为96．3％。结论微流控芯片系统是一种快速、耗费小、可重复使用的AFP测定方法。
Objective To establish a microfluidic chip system for immunoassay. Methods Using direct -write laser micromachining technolique to fabricate microflidic chip, the whole system was constructed by fluid control system, immunoreaction system and signal analysis system. This system was used to detect alpha-fetoprotein. Results The analysis was completed within 20min, 5μl sample and reagent was needed. There was good linear correlation in the range of 1-800ng/ml. Average intra-assay and interassay precision was 4.8% and 7.0 % respectively. Average recovery was 96.3%. Conclusion The microfluidic chip system is a time-saving, cost-low and convenient method for AFP analysis.
出处
《重庆医学》
CAS
CSCD
2006年第6期510-511,514,共3页
Chongqing medicine
关键词
磁珠
化学发光
微流控芯片
免疫分析
magnetic microbeads
chemiluminescent
microfluidic chip
immunoassay
