摘要
选择得油率1．4％以上，其樟油含醇量96％以上、芳樟醇含量93％以上、樟脑含量低于0．1％的三年生香樟，以当年生嫩枝为外植体进行快繁技术研究。结果表明：无菌活体获取率最高的是每年2、3月接种的，1月接种的次之；筛选出适宜香樟组织培养的基本培养基为MS2[MS＋Ca（NO3）2·4H2O 200mg／L]；继代芽培养理想的激素组合为6-BA3．0～3．5mg／L＋IAA2．0mg／L，继代周期25～30d，芽增殖3．5倍，年繁殖数5．31×10^5；MS2＋IBA3．0mg／L＋NAA1．5mg／L＋氯化胆碱50～100mg／L是不定根诱导的适宜培养基，生根率达86．7％；组织培养再生檀株移栽成活率最高的基质是塘泥，成活率达85％。
Selected twigs of 3 years＇ Cinnamomum camphora （The rate of oil was over 1.4%, the ethanal content of the oil was over 96%, which over 93% was linalool and the content of camphor was under 0. 1 % ） as explants to research the rapid propagation and tissue culture. The result showed that the survival rate of sterile twigs was highest in March and February and followed by January; The basic medium for camphor tissue culture was MS2 [MS ＋ Ca （NO3）2 · 4H2O 200 mg/L]; The hormone of basic sub-culture medium was 6-BA 3.0-3.5 mg/L＋IAA 2. 0 mg/L,the period of sub-culture was 25-30 d, the number of multiplication was 3.5 times, the coefficient of multiplication inone year was 5.31×10^5. The medium for rooting was MS2＋IBA 3.0 mg/L＋NAA 1.5 mg/L＋ choline chloride 50 100 mg/L,the rate of rooting was 86.7%. The best soil for transplant was from tank which survival rate after transplant Was 85%.
出处
《广西农业生物科学》
CSCD
2006年第1期60-64,共5页
Journal of Guangxi Agricultural and Biological Science
基金
广西科技厅科技攻关项目(桂科攻9820013)
关键词
香樟
优良无性系
快速繁殖
Cinnamomurn carnphora
elite clone
rapid propagation
