摘要
目的：对西洋参叶皂苷碱降解物中20（S）-人参皂苷Rh2进行含量测定。方法：采用RP-HPLC，ZORBAX EXTEND C18柱（4．6mm×250mm，5μm），流动相甲醇-水（85：15），流速1．2mL·min^-1，检测波长203nm，柱温25℃。结果：20（S）-人参皂苷Rh2的进样量在0．5～25μg有良好的线性关系，r=0．9999；平均加样回收率为99．7％，RSD1．0％。结论：该方法测定20（S）-人参皂苷Rh2简便快捷，准确度高，测定结果证实该降解方法20（S），人参皂苷Rh2的转化率高。
Objective： To determine 20（ S）-ginsengnoside Rh2 in the hydrolysis product of saponins from leaves of Panax qinquefolium. Method： The separation was performed on ZORBAX EXEND C18 column（4.6 mm × 250 mm,5μm）, eluted with methanol and water （85：15） as mobile phase with the rate of 1.2 mL·min^-1 at 25℃, the wavelength for measurement was 203 nm. Resuit： The calibration curve was linear in the range of 0.5-25 μg for 20（S）-ginsengnoside Rh2 （ r = 0.999 9, n = 7）. 1he average recovery was 99.7 % （RSD = 1.0% ）. Conclusion： This method is simple, accurate, reliable and reproducible. The result shows that the transform ratio of 20（ S）- ginsengnoside Rh2 is high by this hydrolysis method.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第5期386-388,共3页
China Journal of Chinese Materia Medica
