摘要
目的优化大肠杆菌表达重组蛇毒锯鳞蝰素(Echistatin,Ecs)融合基因工程菌的发酵工艺。方法在15L发酵罐内,研究培养基、培养条件和诱导时间对工程菌生长和目的蛋白表达的影响,并考察工程菌中重组质粒的遗传稳定性。结果工程菌在pH7·4的2×YT培养基中诱导4h,菌体湿重可达75g/L,目的蛋白表达量约占总蛋白的35%,所构建的重组质粒在BL21宿主菌中传代稳定。结论优化了Ecs融合基因工程菌的发酵和表达条件,为规模化生产奠定了基础。
Objective To optimize the fermentation procedure of recombinant E. coli strain expressing Echistatin fusion gene. Methods Study the influence of medium and time for incubation and induction on the growth of recombinant E. coli and expression of target protein in a 15 L fermentor, and explore the genetic stabihty of recombinant plasmid. Results After the recombinant E. coli strain cultured in 2 × YT medium （ pH 7.4） was induced for 4 h, the wet weight of bacteria reached 75 g/L. The expressed product contained about 35% of total somatic protein. The constructed recombinant plasmid was inherited steadily in host bacterial strain E. coli BL21. Conclusion The condition for fermentation of recombinant E. coli strain and expression of Echistatin fusion gene was optimized. It laid a foundation of large-scale production of Echistatin protein.
出处
《中国生物制品学杂志》
CAS
CSCD
2006年第1期84-86,共3页
Chinese Journal of Biologicals
基金
山西省科技攻关项目(051100-8)
山西省科学技术发展计划项目(042035)资助
