摘要
目的:探讨南通地区乙肝病毒(HBV)基因型分布状况及其临床相关性。方法:选择南通地区HBV-DNA阳性者200例,其中乙肝表面抗原携带者(ASC)28例,慢性乙型肝炎(CHB)89例,重型肝炎(FHF)26例,肝硬化(LH)30例,肝细胞癌(HCC)27例,采用多对型特异性引物巢式PCR法检测HBV基因型。结果:200例中B型51例(25.5%),C型141例(70.5%),BC混合型8例(4.0%);C基因型在FHF组、LC组、HCC组中的比例显著高于ASC组(P<0.05)。B型、C型、BC混合型HBeAg阳性率分别为49.0%、53.2%和37.5%,无统计学意义(P>0.05)。C型和BC混合型HBV-DNA载量显著高于B型,差异有统计学意义(P<0.05)。结论:南通地区HBV基因型以B、C型为主,C型为优势基因型,并与重型肝炎、肝硬化、肝癌的发生及血清HBV-DNA高载量相关。
Objective： To investigate the distribution of hepatitis B virus genotypes in Nantong and its clinical significance. Methods： Serum samples from 200 HBV-DNA positive patients with chronic HBV infection, including 28 asymptomatic HBV carriers（ASC）, 89 chronic hepatitis B（CHB）, 26 fulminant hepatic failure（FHF）, 30 liver cirrhosis（LC）, and 27 hepatocellular carcinoma（HCC） patients were collected and determined for HBV genotypes by PCR with genotype-specific primers. Results： Of the 200 samples, 51 （25.5%） were genotype B and 141（70.5%） were genotype C, 8（4.0%） were mix-infected by genotype B ＆ C. Genotype C was more prevalent in the FHF, LC and HCC patients than in the ASC patients （P〈0.05）. The concentration of HBV-DNA was higher in genotype C and B C mixed than in genotype B（P〈0.05）. There were no statistical differences in the rate of HBeAg positive in genotype B, C ＆ B C mixed（P〉 0.05）. Conclusions： The dominant genotype of HBV in Nantong is genotype C; Genotype C is associated with the development of severe liver diseases.
出处
《中国交通医学杂志》
2006年第1期49-50,53,共3页
Chinese Medical JOurnal of Communications
