摘要
目的:探讨3种溶血磷脂酸(lysophosphatidic acid,LPA)受体(LPA1,LPA2及LPA3)mRNA和蛋白在人卵巢肿瘤组织中的表达状况及意义。方法:采用半定量RT-PCR和Western blot检测LPA1、LPA2和LPA3mRNA以及LPA2和LPA3蛋白在人卵巢上皮性肿瘤组织中的表达水平。结果:RT-PCR结果显示,LPA1mRNA在正常卵巢组织、良性卵巢肿瘤及上皮性卵巢癌中表达阳性率分别为100%、100%、95.7%,差异均无显著性(P>0.05);上皮性卵巢癌组织LPA2mRNA、LPA3mRNA表达阳性率(95.7%;91.3%)显著高于良性卵巢肿瘤(21.3%;21.3%)及正常卵巢组织(15.4%;15.4%)(P<0.01);良性卵巢肿瘤与正常卵巢组织LPA2mRNA、LPA3mRNA表达阳性率差异无显著性(P>0.05);上皮性卵巢癌组织中LPA1mRNA表达率和表达水平高于LPA3mRNA(P<0.05)。Western blot结果显示,上皮性卵巢癌组织中LPA2和LPA3蛋白表达阳性率(95.7%;95.7%)均高于良性卵巢肿瘤(50%;42.9%)和正常卵巢组织表达阳性率(38.5%;38.5%)(P<0.01);良性卵巢肿瘤与正常卵巢组织中LPA2和LPA3蛋白表达阳性率及表达水平相比差异无显著性(P>0.05)。病理资料的相关分析显示,上皮性卵巢癌组织中LPA受体mRNA和蛋白表达与临床病理特征无相关性。结论:LPA1、LPA2和LPA3在卵巢上皮性肿瘤中普遍表达,LPA受体可能参与了卵巢癌的发生发展过程。
Objective： To investigate the lysophosphatidic acid （LPA） receptors expression and their biological significance in human epithelial ovarian neoplasms. Methods： The reverse transcription polymerase chain reation （RT PCR） and Western blot were employed to measure the expression of LPA-1,LPA-2,and LPA 3 receptor mRNA and LPA-2 and LPA-3 receptor protein expression in human epithelial ovarian neoplasms. Results： 1）The positive rate of LPA-1 receptor mRNA in normal ovarian tissue, ovarian benign disease, and ovarian cancer was 100 %, 100 %, and 95.7 %, respectively. The difference was not significant between them（P〉0.05）. The positive rates of LPA-2 and LPA 3 receptor mRNA （95.7 % and 91.3 %） were significantly higher in epithelial ovarian neoplasms than in ovarian benign disease （21.3% and 21. 3%） and normal ovarian tissue （15. 4% and 15.4%）. The positive rates of LPA-2 and LPA-3 receptor mRNA had no significant difference between ovarian benign disease and normal ovarian tissue（P;〉0.05）. The mRNA expression of LPA-1 receptor was markedly higher than LPA 3 receptor in epithelial ovarian neoplasms（P〈0.05）. 2）Western blot revealed that the positive rates and expression level of LPA. 2 and LPA-3 receptor protein were significantly increased in epithelial ovarian neoplasms compared with ovarian benign disease and normal ovarian tissue （95.7% and 95.7% vs 38.5% and 38.5% ,P〈0.01）. The positive rates and expression level of LPA-2 and LPA- 3 receptor protein had no significant difference between ovarian benign disease and normal ovarian tissue（P〉0.05）. 3）No significant correlation was found between LPA receptor expression and clinicopathological features. Conclusion： LPA1, LPA2, and LPA-3 receptors were highly expressed in human epithelial ovarian neoplasms. LPA receptors may be involved in the development and progression of human ovarian cancer.
出处
《肿瘤》
CAS
CSCD
北大核心
2006年第3期289-292,305,共5页
Tumor
关键词
卵巢肿瘤
溶血磷脂酸
受体
基因表达
Ovarian neoplasms
Lysophosphatidie acid
Receptor
Gene expression
