摘要
目的通过检测组织中基质金属蛋白酶-2(MMP-2)和基质金属蛋白酶抑制剂-2(TI MP-2)的表达,探讨它们在子宫内膜异位症发生、发展中的作用。方法用原位杂交染色技术对MMP-2和TI MP-2mRNA进行测定。结果①MMP-2在异位内膜的表达高于在位内膜(P<0·05);在位内膜MMP-2的表达显著强于正常子宫内膜(P<0·05),而且异位组中Ⅲ、Ⅳ期组与Ⅰ、Ⅱ期组间差异有统计学意义(P<0·05)。TI MP-2在位内膜的表达低于正常子宫内膜(P<0·05),异位内膜中TI MP-2的表达显著低于在位内膜(P<0·05),而且异位组中Ⅲ、Ⅳ期组与Ⅰ、Ⅱ期组间差异有统计学意义(P<0·05)。②在正常内膜中,MMP-2与TI MP-2之间呈负相关,r=-0·710。结论患者组织中MMP-2和TI MP-2的异常表达在子宫内膜异位症的发生、发展中起重要的作用。
Objective To investigate the expression of MMP-2 and TIMP-2 in affected tissue from women with and without endometriosis for exploring the role of MMP-2 and TIMP-2 in endometriosis. Methods Expression of MMP-2 and TIMP-2 mRNA was evaluated by in situ hybridization. Results ①Both ectopic and entopic endometrium expressed higher levels of MMP-2 and lower levels of TIMP-2 than endometrium from normal women. Also, ectopic endometrium expressed higher levels of MMP-2 and lower level of TIMP-2 than entopic endometrium from endometriosis patients. The higher levels of MMP-2 and the lower levels of TIMP-2 expressed in stages Ⅲ/Ⅳ than in stages Ⅰ/Ⅱ. ②There was a significantly negative correlation between expression of MMP-2 and TIMP-2 in normal endometrium. Conclusion MMP-2 and TIMP-2 play an important role in the progression of endometriosis.
出处
《山西医药杂志》
CAS
2006年第3期202-203,T0001,共3页
Shanxi Medical Journal
