摘要
目的:探讨非小细胞肺癌(NSCLC)中神经内分泌(NE)分化与多药耐药(MDR)的相关性。方法:采用链霉亲和素过氧化酶连接(SP)免疫组化法检测113例NSCLC中神经元特异性烯醇化酶(NSE)、突触素(SYN)和嗜铬素A(CgA)的表达,采用原位分子杂交对同一病例中多药耐药基因(MDR1)和多药耐药相关蛋白(MRP)基因mRNA的表达进行检测,并对相关数据进行统计学处理。结果:①NSCLC中NSE、SYN、CgA的阳性率分别为53.1%,26.6%和6.2%。至少有2种NE标记物表达者有24例(21.2%),其表达与肿瘤的分化程度有关(P<0.05);②MDR1和MRPmRNA在NSCLC组织中的阳性表达率分别为51.3%,80.5%,二者表达与肿瘤组织类型、分化程度、淋巴结转移、TNM分期等无关,二者在NSCLC中的表达存在明显相关(P<0.01);③2种MDR相关基因mRNA在具有NE分化的NSCLC中的表达均较不伴NE分化者为低(P<0.05)。结论:NE分化可能与NSCLC多药耐药性的产生有密切关系。
Objective： To investigate the correlation between neuroendocrine （NE）differentiation and multidrug resistance （MDR） in （non-small cell lung cancer, NSCLC）. Methods： The expression of NSE,SYN and CgA were measured by immunohistochemistry. In situ hybridization was used to detect MDR1 and MRP gene. The results were checked with x^2 test. Results. ① The positive rates of the NE markers were 53.1%, 26.6% and 6.2% for NSE, SYN and CgArespectively, and 21.2% for at least two markers positively expressed. The expression of at least two markers was associated with the degree of differentiation （P〈0.05）. ②Fifty-eight cases （51.3%） were MDR1 mRNA-positive and nighty-one cases （80.5 %） were MRP mRNA-positive respectively. The expression of MDR1 mRNA and MRP mRNA was not related to histological subtypes, cell differentiation, TNM stages and lymphatic metastasis. There was a significant correlation between expression of MDR1 mRNA and MRP mRNA in NSCLC （P〈0.01）. ③ The expression of the two MDR associated genes in the positive group for at least two markers was significantly lower than that in the negative one （P〈0.05）. Conclusion： There may be a close relationship between NE differentiation and MDR in NSCLC.
出处
《武汉大学学报（医学版）》
CAS
2006年第2期232-234,共3页
Medical Journal of Wuhan University
基金
湖北省自然科学基金(编号:2002AB145)
湖北省科技攻关计划项目(编号:2002AA304B13)
关键词
肺肿瘤
神经内分泌
多药耐药
免疫组织化学
原位分子杂交
Lung Neoplasms
Neuroendocrine
Multdrug Resistance
Immunohistochemistry
In Situ Hybridization
