摘要
目的:探讨细胞色素P4501A1(CP1A1)和载脂蛋白E基因多态性及其交互作用与阿尔茨海默病的关系。方法:实验于2004-11/2005-05在南方医科大学珠江医院中心实验室进行。应用聚合酶链反应-限制性片段长度多态性方法检测了135例散发性阿尔茨海默病患者和138例正常老年人细胞色素P4501A1基因和载脂蛋白E基因多态性分布特征。结果:阿尔茨海默病组135例,健康对照组138例,均进入结果分析。①细胞色素P4501A1等位基因m1,m2频率:散发性阿尔茨海默病组分别为63%,37%,对照组分别为68.1%,31.9%,各等位基因频率分布差异无显著性(χ2=1.605,P>0.05)。②散发性阿尔茨海默病组载脂蛋白Eε4等位基因频率与对照组比较有统计学意义(10.74%,5.80%,χ2=4.41,P<0.05,OR=1.95),散发性阿尔茨海默病组和对照组各基因型分布差异无统计学意义(P>0.05)。③研究对象按携带载脂蛋白Eε4状况分层后,在带有ε4等位基因的人群中,散发性阿尔茨海默病组细胞色素P4501A1m2等位基因频率与对照组比较差异有显著性意义(7.4%,2.2%,χ2=6.825,P=0.009),患病风险增加3.417倍(OR=3.417)。结论:细胞色素P4501A1基因MSP1多态性与中国汉族人散发性阿尔茨海默病发病无明显关联,但在携带有载脂蛋白ε4基因型的个体,细胞色素P4501A1m2等位基因可能是散发性阿尔茨海默病的危险基因。
AIM： To assess the association between Alzheimer disease and the polymorphisms of cytochrome P4501A1 gene （CYP1A1） and apolipoprotein E gene （APOE）.
METHODS： The experiment was conducted in the central laboratory, Zhujiang Hospital of Southern Medical University between November 2004 and May 2005. The distributive characteristics of the polymorphisms of CYP1A1 and apolipoprotein E gene were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 135 patients with sporadic Alzheimer disease and 138 normal elderly people.
RESULTS： All the 135 cases in the sporadic Alzheimer disease group and 138 cases in the normal control group were involved in the analysis of resuits. （1） The frequencies of CYP1A1 alleles of ml and m2： The distributions of the allele frequencies had no significant differences between the sporadic Alzheimer disease group and control group [63%, 37%; 68.1%, 31.9%; χ^2=1.605, P 〉 0.05]. （2） The frequency of the apolipoprotein E 84 allele was significantly different between the sporadic Alzheimer disease group and control group （10.74%, 5.80%, χ^2 = 4.41, P 〈 0. 05, OR=1.95）, and the frequency of apolipoprotein E genotypes had no significant difference （P 〉 0.05）. （3） After the stratification by apolipoprotein E ε4, significant interaction was found between CYP1A1 m2 allele and apolipoprotein E allele between patients with sporadic Alzheimer disease and the control group （7.4%, 2.2%, χ^2 = 6.825, P =0.009）, find prevalence risk was increased by 3.417 times （OR =3.417）.
CONCLUSION： MSP 1 polymorphism of the CYPIA1 gene is not associated with sporadic Alzheimer disease in Chinese Han population, but m2 allele of CYP1A1 gene may be a risk allele associated with the development of Alzheimer disease of apolipoprotein E ε4 allele.
出处
《中国临床康复》
CSCD
北大核心
2006年第10期107-109,共3页
Chinese Journal of Clinical Rehabilitation
