摘要
将乙酰胆碱酯酶和牛血清白蛋白通过戊二醛共价交联，固定在氢离子敏场效应管的栅极上，制成胆碱酯酶场效应管传感器。采用差动检测方式，用两只传感器可测出10－7mol／L的敌敌畏等有机磷农药。线性范围为5×10－7～8×10－6mol／L，具有较强的抗干扰能力，寿命为20d以上。
By crossliking with glutaraldehyde, acetylcholine esterase and bovine serum albumin are directly immobilized on the surface gate of a H+-ion-selective field-effect biosensor.In the mode of differential-signal determination, use of two biosensors makes it possible todetect organophosphorus insecticides such as dichlorvos at the level of 10-7 mol/L, with a linear range of 5 × 10-7~8 × 10-6 mol/L. This method has the advantages of stable performance and strong anti-interference ability. In the PB-buffer its life is as long as 20 days.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1996年第5期521-524,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
贵州省科学基金
关键词
胆碱酯酶
场效应管
有机磷农药
生物传感器
Choline esterase, ion selective field effect transistor, organophosphorus insecticide, biosensor
