摘要
采用活性炭吸附与海藻酸钙凝胶包埋相结合的方法使Coriolus versicolor漆酶固定化．利用固定化漆酶对2，4-二氯酚进行脱氯反应，其最适pH值为4．5、最适温度为40℃．与游离酶相比，固定化酶反应的pH值和温度范围更宽，其稳定性得到了明显改善．使用柱式固定化酶反应器处理2，4-二氯酚，在批式反应工艺条件下，当底物浓度为1mmol·L^-1、反应3～5h，2，4-二氯酚的去除率可达99．5％以上（脱除的氯离子浓度达0．5mmol·L^-1）-连续8批反应的结果表明：固定化漆酶性能稳定、催化效率高，在环境污染废水治理方面具有良好的应用前景．
Laccase from Coriolus versicolor was immobilized by adsorbing first on activated carbon, and then entrapping into calcium alginate gels. 2,4-Dichlorophenol in wastewater was dechlorinated by the immobilized laccase in a columnar reactor. The optimal temperature and pill for the reaction were 40℃ and 4.5, respectively. Compared with free laccase, the immobilized laccase bad better thermostability, and also had a broader active range of pH and temperature. The decholorination efficiency of laccase on 2,4- dichlorophenol （1 mmol · L^-1） was all above 99.5% with 0.5 mmol · L^-1 chloride ions released into the water during 8 repeated batch reactions. The results showed good prospect of application in the biochemical treatment of polluted wastewater.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第2期359-362,共4页
CIESC Journal
关键词
固定化
漆酶
2
4-二氯酚
脱氯
废水
治理
immobilization
laccase
2,4-dichlorophenol
dechlorination
wastewater
biochemical treatment
