摘要
目的了解小肠结肠炎耶尔森菌(Yersinia enterocolitica,Yen)强毒力岛在肠聚集性大肠杆菌(EAggEC)中的结构和摄铁功能,为进一步研究细菌毒力的进化奠定理论基础。方法PCR扩增、原位杂交及SDS-PAGE。结果在检测的6株EAggEC有5株检出了毒力岛,而且这些阳性菌株中的毒力岛都位于asntRNA(天门冬氨酸tRNA)位点;在缺铁条件下,EAggEC能够表达和耶尔森菌相同的摄铁蛋白HMWP1和HMWP2。结论小肠结肠炎耶尔森菌强毒力岛在肠聚集性大肠杆菌中有阳性率较高的分布,并具有相同的摄铁功能,很可能EAggEC和耶尔森菌中的强毒力岛有相同的转移机制。
Objective To detect the ＂high pathogenicity island＂（HPI） of Yersinia enterocolitica（Yen） WA in enteroaggregative E. coli, as the theoretical base of bacterial evolution process and the different structure of E.coli. Methods Polymease chain reaction（PCR）, nucleic acid hybridization in situ, DNA sequencing and SDS-PAGE. Results Five strains were isolated from 6 EAggEC strains, in these isolates, the HPI bordered by an asntRNA locus, as in Yersinia spp, at the condition of iron deficiency, EAggEC expressed two high molecular weight proteins, HMWP1 and HMWP2. Conclusion The HPI of Yen was found in EAggEC, and the HPI which habored the asntRNA locus has full structure and same iron uptake function as Yersinia, maybe the HPI of EAggEC and Yen shares a same mechanism of transfer.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第2期159-162,共4页
Chinese Journal of Microbiology and Immunology
关键词
大肠杆菌
耶尔森菌
强毒力岛
摄铁功能
E. coli
Yersinia enterocolitica
High pathogenicity island
Iron-uptake function
