摘要
目的研究电转染技术结合初免后加强免疫策略能否增强结核杆菌核酸疫苗的免疫原性。方法将编码结核杆菌的Ag85A和ESAT-6蛋白抗原的基因分别插入到质粒pVAX1载体中，构建成HG85和HG6两种核酸疫苗。每只BALB／c小鼠肌肉注射10μg核酸疫苗，同时于注射部位施加方型波电脉冲促进质粒DNA体内转染；进行3次初免，再用相应蛋白质抗原或卡介苗加强免疫。结果肌肉注射10μg核酸疫苗加电转染能诱导出与不用电转染接种100μg核酸疫苗相似或更强的抗体免疫应答。初免后采用相应蛋白加强后小鼠产生的免疫应答偏向于TH2型，血清特异性抗体的几何平均滴度比加强前提高5—76倍。而用卡介苗加强免疫，产生的免疫应答偏向TH1型。结论采用电转染技术结合蛋白质或卡介苗加强免疫策略，能显著增强结核杆菌核酸疫苗在动物体内的免疫原性。
Objective A study for the enhancement of immunogenieity of tuberculosis DNA vaccines by electroporation （EP） prime and boost strategy. Methods DNA vaccines HG85 and HG6 were constructed by inserting the gene encoding Ag85A or ESAT-6 into pVAXl vector respectivdy. BALB/c mice were intramuscularly injected with 10μg HG85 or HG6 DNA vaccine followed by electroporation. Electroporation of plusmid DNA in vivoo was improved by square wave applied at the site of DNA injection. Mice were DNA/EP primed for three times and boosted with corresponding recombinant protein or BCG. Remits A htanoral immunity induced by injecting mice with 10μg DNA followed by EP was equal to or better than that obtained by using 100 μg DNA without EP. Boosting the DNA/EP primed animals with corresponding recombinant protein induced TH2 immune response and evoked more than a 5-76 fold increase in the antibody titer. However, boosting the DNA/EP primed animals with BCG induced TH1 immune response. Cluclusion The immunogenieity of tuberculosis DNA vaccines in animals could be significantaly increased by DNA/EP prime and protein or BCG boost strategy.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第2期127-131,共5页
Chinese Journal of Microbiology and Immunology
基金
上海市科委课题资助(编号:03DZ19230
045458036)
卫生部科教司基金资助
关键词
结核病疫苗
体内电转染
DNA初免和加强免疫策略
免疫原性
Tuberculosis DNA vaccines
Electroporatio in vivo
DNA vaccines prime-boost strategy
Immunogenicity
