摘要
目的:探讨碱性成纤维细胞生长因子(basicfibrob-lastgrowthfactor,bFGF)对体外培养牛视网膜色素上皮(retinalpigmentepithelium,RPE)细胞特异性吞噬功能的影响及实验方法的选择。方法:采用辣根过氧化物酶Ⅱ型(horseradishperoxi-diase,HRP)示踪法测定不同bFGF浓度及作用时间对体外培养单层牛RPE细胞吞噬HRT量的影响。结果:不同bFGF浓度及作用时间各组光吸收值差异均不显著。结论:bFGF对体外培养牛RPE细胞特异性吞噬功能无明显影响。
AIM： To investigate the effects of bFGF on phagocytosis of cultured bovine retinal pigment epithelium cells in vitro.
METHODS： The effect of varied bF-GF concentration and working time on phagocytosis capacity of cultured bovine RPE was detected by the HRP tracing method.
RESULTS： The phagocytosis tests of HRP indicated that bFGF had no effect on the light absorption whatever its concentration or working time was.
CONCLUSION： bFGF has no effect on the specific phagocytosis of cultured newborn bovine RPE cells in vitro.
出处
《国际眼科杂志》
CAS
2006年第1期72-74,共3页
International Eye Science
基金
"九五"军队医药卫生科研基金资助项目(No.96M143)
