摘要
目的:观察表皮生长因子(epidermalgrowthfactor,EGF)对培养的人视网膜色素上皮(retinapigmentepitheliumcell,RPE)细胞中缝隙连接蛋白Cx43蛋白及mRNA的表达影响。方法:不同浓度的EGF作用于培养的第4代人的RPE细胞24h,采用Westernblot的方法测定Cx43的蛋白;原位杂交观察Cx43mRNA在RPE细胞中的表达特点;RT-PCR实验对EGF影响后的RPE细胞的mRNA进行半定量观察。结果:Westernblot试验证实不同浓度的EGF均可使Cx43蛋白表达量明显减少,并且与EGF的浓度呈正相关,原位杂交显示所有细胞均表达Cx43mRNA,其表达部位主要在细胞质和核;RT-PCR实验对mRNA进行半定量观察显示表皮生长因子使RPE细胞的mRNA表达量明显减少。结论:EGF可以下调Cx43蛋白及Cx43mRNA在人RPE细胞中的表达。
AIM： To investigate the effects of Epidermal growth factor （EGF） on the degree of connexin43 （Cx43） protein and mRNA expression in cultured human retinal pigment epithelial （RPE） cells.
HETHODS： Western blot experiment was used to determine the protein express after RPE cell was incubated under different concentration of EGF 24h; in situ hybridization of Cx43mRNA was performed in RPE cells. RT-PCR was carried out for mRNA half quantity.
RESULTS： Western blot experiment showed that EGF induced protein express decreased; in situ hybridization of Cx43mRNA indicated that mRNA expressed meanly in cell nucleus; RT-PCR test showed that EGF made mRNA of RPE cell express also decreased significantly.
CONCLUSION： EGF can downregulate the level of Cx43 protein and mRNA express in human RPE cell.
出处
《国际眼科杂志》
CAS
2006年第1期57-60,共4页
International Eye Science
基金
中国北京市自然科学基金资助(No.7032046)
