摘要
首次报道了大黄鱼诺卡氏菌病的发生情况。病鱼以体表和心、脾、肾等内脏出现白色结节为主要症状,平均死亡率15%。对病鱼进行细菌分离培养和光镜、电镜检查,均发现长或短的丝状分枝状杆菌。用分离菌株作回归感染,证实为该大黄鱼结节病的病原菌。对病原菌的基本生物学特性进行了研究,确定病原为诺卡氏菌。
An epizootic in seawater cage-reared large yellow croaker, Pseudesciaena crocea, in China from September to November 2003 was caused by a Nocardia sp. The cumulative mortality was 15% and diseased fish were 16 months old with total length from 20 to 30 cm. Multiple, white nodules, 0.1 - 0.3 cm in diameter, were scattered on the heart, spleen and kidney. The morphology of isolated bacteria from Lowenstein-Jensen medium （LJM） and trypfic soy agar （TSA） were Gram-positive, branching, bead-like or long, slender, filamentous rods. Experimental infection indicated that the isolated bacterium was pathogen responsible for the mortalities and the bacterium colonizes the fish via skin or gill, and intragastric exposure to the pathogen was less effective as a route of infection. Based on the symptom of diseased fish and the growth characteristics, morphological, physiological, and biological properties of the bacterium, the isolated were identified as Nocardia sp.
出处
《水产学报》
CAS
CSCD
北大核心
2006年第1期103-107,共5页
Journal of Fisheries of China
基金
浙江省自然科学基金项目(Y304078)
浙江省教育厅项目(20051691)
