摘要
目的: 探讨地塞米松(dexam ethasone,D EX)对培养的兔角膜上皮细胞和角膜上皮伤口愈合的影响。方法: 体外实验原代培养兔角膜上皮细胞, 用四唑盐(M TT)实验和流式细胞仪检测不同浓度 D EX 对兔角膜上皮细胞的作用, 同时通过 H E 、免疫组织化学染色和扫描电镜及临床观察兔角膜上皮细胞刮除后 0.1g/L D EX 用药组和对照组伤口愈合的情况。结果: M TT 检测显示小于 0.1g/L 浓度的 D EX 对兔角膜上皮细胞的生长无明显影响。流式细胞仪检测到 0.1g/L 浓度的 D EX 对兔角膜细胞生长周期无抑制作用。动物实验: 0.1g/L 地塞米松作用后上皮愈合时间显著性缩短, 两组中新生成的上皮细胞均有较强的 PC N A 蛋白的表达。0.1g/L 地塞米松组与对照组相比, 电子显微镜检查角膜基质排列更规则, 炎症反应较轻。结论: 低于 0.1g/L 浓度的地塞米松对培养的兔角膜上皮细胞的生长无抑制作用。0.1g/L 浓度的地塞米松眼液能有效的促进角膜上皮生长并降低炎症反应, 将有利于准分子激光角膜屈光手术后早期角膜伤口愈合。
AIM： The study was undertaken to investigate the effort of Dexamethasone （DEX） on cultured rabbit corneal epithelial （RCE） cells and rabbit corneal epithelial wound healing.
METHODS： For the in vitro experiments, primary cultures of RCE cells were used. DEX in different concentrations was added to cultured RCE cells. The effects were measured with tetrazolium salt （MTT）method and flow cytometry. For the in vivo wound-healing experiments, a central corneal deepithelialization was created and were treated with 0.1g/L DEX eyedrop randomly explain how randomly. Epithelial wound healing was evaluated clinically and analyzed histopathologically using light microscopy along with immunohistochemical staning and electronic microscopy.
RESULTS： Less than 0.1g/L DEX didn＇t influence survival rate in cell culture conditions by MTT assay. Flow cytometric studies revealed that 0.1g/L DEX had no effect on cellular growth phase in cultured rabbit corneal epithelial cells. The mean time of the epithelial healing was significantly shorter in the DEX-treated group than in the control group at 24h. There were strong proliferative-cell-nuclear-antigen（PCNA） expressions in newly generated epithelial cells of both groups. The Dex-treated group had a more regular architecture of stromal lamella and significantly less inflammatory response than the control group under electronic microscopy.
CONCLUSION： Less than 0.1g/L DEX had no inhibiting effect on cultured rabbit corneal epithelial cell growth. 0.1g/L DEX eye drops can effectively promote epithelial growth and reduce inflammatory response, which may have useful clinical application at the early stage of corneal wound healing process.
出处
《国际眼科杂志》
CAS
2006年第1期1-4,共4页
International Eye Science
关键词
角膜上皮细胞
地塞米松
兔
免疫组化
电镜
corneal epithelial cell
dexamethasone
rabbit
immunohistochemistry, electronic microscopy
