摘要
目的研究内毒素(ET)致大耳白兔急性肺损伤(ALI)过程中磷脂酶A2的变化和1,6-二磷酸果糖(FDP)的拮抗作用,探讨FDP对ET所致ALI的治疗作用。方法大耳白兔随机分为对照组(A组)、ET致伤组(B组)、ET致伤+FDP干预组(C组)。A组:静脉注射生理盐水2ml/kg。B组:将内毒素按500μg/kg溶于2ml生理盐水后经颈静脉插管一次注射完毕,随后注射生理盐水1次,液体总量为2ml/kg。C组:内毒素使用剂量和方法同致伤组,随后缓慢经静脉注射FDP(300mg/kg)的生理盐水溶液,液体总量同为2ml/kg。各组分别于0h、0·5h、2h、4h及6h等不同时点测定呼吸频率、心率、血压、血气分析及血清中PLA2活性;于6h点处死动物,测定肺组织PLA2活性,并行肺组织光镜和电镜下病理学观察。结果静注ET后,B组动物与A组相比较,出现典型的ALI表现,血清和肺组织PLA2活性显著增高(P<0·01),而C组动物各项指标则介于A组和B组之间。同时,肺组织病理学观察显示,B组动物肺组织出现了明显的病理损害,而C动物肺组织的病理损害程度较轻。结论PLA2激活是ET所致ALI发病过程中重要的致伤因素。FDP可抑制PLA2激活,从而对ET所致的兔早期ALI有一定的保护作用。
Objective To study the changes in activity of phospholipase A2 （PLA2） in the course of endotoxin （ET）-induced acute lung injury （ALI） in rabbits and the antagonizing effect of fructos-1,6- diphosphate （FDP）. in order to evaluate the therapeutic effect of FDP on ET-induced ALI Methods Flapeared white rabbits were randomly assigned to three groups： control group （group A）. ET challenge group （group B） and treatment group （ET challenged followed by FDP. group C）. Group A animals were injected with ,saline （2ml/kg） as control. Group B animals were injected with ET （500μg/kg） ,solution followed by ,saline. Total amount of liquid was 2ml/kg. Group C animals were given the ,same amount of ET ,solution followed by injection of FDP （300mg/ kg） ,solution. Total amount of liquid was also 2 ml/kg. During the experiment, respiratory rate. heart rate. blood pressure, arterial blood gases and the plasma PLA2 activity were determined at Oh, 0. 5h. 2h, 4h and 6h respectively. The rabbits were ,sacrificed at 6h, pulmonary PLA2 activity was assessed, and the pathologic changes in pulmonary tissues were examined with light microscope and transmission electron microscope. Results Compared with group A, rabbits of group B manifested the typical characters of ALI after ET injection, and the PLA2 activity in both serum and pulmonary tissue was much higher than those of group A （P〈0. 01）. Values of the PLA2 activity in group C were between those of the two former groups. At the ,same time, obvious pathological changes indicating lung injury were observed in group B and only mild pathological changes could be discerned in group C. Conclusion Activation of PLA2 activity is an important factor in pathogenesis of ET-induced ALI. FDP can antagonize the PLA2 activity and protect rabbits from early ET-induced ALI to a certain extent.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2006年第3期192-195,共4页
Medical Journal of Chinese People's Liberation Army
基金
Supported by military medicine and sanitation scientificresearch fund, No. 00J02
关键词
果糖二磷酸盐类
呼吸窘迫综合征
成人
磷脂酶
A
内毒素类
兔
fructose diphosphates, respiratory distress syndrome, adult
phospholipase A
endotoxins, rabbits
