摘要
目的:检测大鼠单侧输尿管梗阻(UUO)模型不同时期,肾组织中结缔组织生长因子(CTGF)、转化生长因子β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)的表达,观察比较在间质纤维化不同阶段,3者的动态变化及关系。方法:采用雄性SD大鼠36只,分为假手术组和模型组,模型组行左侧输尿管结扎术,再分3、7、14、21和28d共6组,每组6只,于各时点处死大鼠,取肾组织,常规HE、Masson染色,按小管间质损害的特征进行半定量评分。免疫组化检测CTGF、TGF-β1和α-SMA表达。结果:随梗阻时间的延长,小管间质纤维化加重,28d间质已基本被纤维化组织所代替。随间质纤维化程度的加重,CTGF和α-SMA表达逐渐增加,两者与小管间质损害积分呈正相关,CTGF与α-SMA的表达之间也呈正相关。TGF-β1表达在7-14d达高峰后,逐渐减少,但仍高于对照组。结论:UUO致CTGF表达增加可能与TGF-β升高有关,CTGF可能通过促进间质中肌成纤维细胞的形成而参与肾间质纤维化。
AIM： To detect the expression of connective tissue growth factor （CTGF）, transforming growth factor- β1 （TGF - β1 ） and α - smooth muscle actin （α - SMA ） in rat unilateral ureteral obstructive （ UUO ） nephropathy animal model, and to observe the kinetic changes at different stages of firosis. METHODS： Male SD rats were subjected to either left ureteral ligation or sham operation, then killed at 3, 7, 14, 21 or 28 days after UUO or sham operation （ n = 6 at each time point）. HE, Masson or PAS staining were applied to the renal tissue sections. The extent of tubulointerstitial injury was determined by Banff classification. RESULTS： The extent of tubulinterstitial fibrosis became serious with the time of obstruction. Tubules were mostly atropic and replaced by proliferative fibrous tissue at day 28. The expression of CTGF and α - SMA were consistent with the damage of tubulointerstitial. The positive correlation among CTGF or α - SMA and the tubulointerstitial injury scores were significant. The expression of TGF- β1 came to peak at day 7 to 14, and gradually decreased at day 21 and 28. CONCLUSION： These results indicate that the expression of CTGF may be upregulated by TGF -β in UUO rats, and CTGF may be involved in tubulointerstitial fibrosis through the development of myofibroblasts.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期427-430,共4页
Chinese Journal of Pathophysiology
基金
中山大学"211工程"资助项目(No.98151)
关键词
结缔组织生长因子
转化生长因子Β
平滑肌肌动蛋白
肾
Connective tissue growth factors
Transforming growth factor beta
Smooth muscle actins
Kidney
