摘要
目的研究人单核细胞源性巨噬细胞向泡沫细胞分化过程中MaxiK通道α-亚单位的表达。方法采用密度梯度离心法从男性健康志愿者外周血中分离单核细胞,经培养后分化为巨噬细胞,并通过加氧化型低密度脂蛋白(OxLDL),建立人巨噬细胞源性泡沫细胞模型,采用RT-PCR、蛋白质印迹及免疫细胞化学方法研究MaxiK通道α-亚单位的表达。结果巨噬细胞同30mg/LOxLDL孵育60h后,细胞内总胆固醇(TC),游离胆固醇(FC)及胆固醇酯(CE)显著增加,并且CE/TC从(14.437±6.781)%提高到(57.946±3.507)%。同时,MaxiK通道α-亚单位表达被下调,但同巨噬细胞相比差异无统计学意义(P>0.05)。结论人单核细胞源性巨噬细胞同30mg/LOxLDL孵育60h后可分化为泡沫细胞,但MaxiK通道α-亚单位的表达无明显改变。
Objective To investigate the expression of MaxiK channel α-subunit during human monocyte-derived macrophages differentiating into foam cells. Methods Human peripheral blood monocytes were isolated from male healthy volunteers by density gradient centrifugation, which, by culture, differentiated further into macrophages as a homogeneous monocyte population. The foam cell model originated from human macrophage was established by incubating macrophages with oxidized low density lipoprotein （OxLDL）. The expression of MaxiK channel α-subunit was investigated by RT-PCR techniques, Western blotting and immunocytochemistry. Results After incubating macrophages with 30 mg/L OxLDL for 60 hours, the cellular contents of total cholesterol （TC）, free cholesterol （FC） and cholesterol ester （CE） were markedly increased and the ratio of CE/TC was further raised from （14.437 ± 6.781）% to （57.946 ± 3.507） %. Although the expression of MaxiK channel α-subunit was downregulated during human monocyte-derived macrophages differentiating into foam cells, there was no significant difference between macrophages and foam cells （ P 〉 0.05）. Conclusion That 30 mg/L OxLDL can lead the monocyte-derived macrophage cultured for 60 hours to differentiate into foam cell, but the expression of MaxiK channel α-subunit does not change obviously.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2006年第2期171-175,共5页
Journal of Sichuan University(Medical Sciences)
基金
纽约中华医学基金会基金(CMB,01-761)
科技部国际合作重点项目(2003DF000037)资助~~
关键词
离子通道
胆固醇代谢
细胞分化
动脉粥样硬化
Ion channel Cholesterol metabolism Cell differentiation Atherosclerosis
