摘要
活性污泥胞外多聚物(EPS)的定量与其提取方法密切相关。基于文献报道的提取方法,采用超声、加热、甲醛加碱等6种方法对活性污泥的EPS进行提取。测定了其中多糖、蛋白质及DNA含量,以评价提取效率及对细胞的破坏程度。结果表明,各种方法提取活性污泥EPS中的蛋白质均多于多糖,且对细胞破坏程度均较小。加热法,甲醛加碱法和超声提取法的提取效率较高,所得多糖和蛋白质含量之和分别为94.30,72.33和56.55mg/gVSS(挥发性悬浮物);通过对不同超声功率和时间的比较,表明在较低功率(25￣50W)超声,提取效率变化不大,最佳超声提取时间为4min。
Extraction methods like sonication,heating,formaldehyde plus sodium hydroxide were applied to extract extracellular polymeric substances(EPS)from aerobic activated sludge.The extraction abilities of these methods were evaluated in terms of extraction efficiency and cells destruction through measuring concentrations of polysaccharide,protein and DNA respectively.Of them,sonication was considered an ideal EPS extraction method.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2006年第3期46-47,53,共3页
Environmental Science & Technology
关键词
胞外多聚物
提取方法
超声提取法
extracellular polymeric substances(EPS)
sonication extraction method
