摘要
目的通过体外代谢获得药物相互作用的有关数据,以预测临床联合用药时发生代谢性药物相互作用的可能性。方法以HPLC测定孵育液中剩余银杏黄酮的浓度,计算共孵育药物硝苯地平对银杏黄酮3个苷元的IC50值和Ki值;以HPLC测定孵育液中硝苯地平的浓度,计算其代谢抑制率。结果硝苯地平与银杏黄酮进行体外共孵育时,代谢互受影响。结论两药合用时宜慎重。
OBJECTIVE To investigate the interaction of nifedipitve with ginkgo flavones in vitro.METHODS Flavonoids quercetin, isorhamnetin, keampferol and nifedipine were co-incubated with rat hepatic microsomal at 25℃ and the concentrations of flavonoids in residue were determined by HPLC. The IC50 and Ki vaules of quercetin, isorhamnetin and keampferol were observed. The conceutrations of nifedipine in residue were determined hy HPLC. Metabolism inhibition rates of nifedipine by ginkgo flavones in rat hepatic microsome were observed. RESULTS Nifedipine interacted with ginkgo flavones in rat hepatic micmsomal in vitro. CONCLUSION The attention should be paid for the interaction when the preparations of nifedipine is taken with ginkgo flavones.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2006年第5期379-381,共3页
Chinese Pharmaceutical Journal
基金
浙江省自然科学基金资助项目(302682)
