摘要
目的探讨广东省马尔尼菲青霉病的分子流行病学情况，比较广东株、广西株和泰国株之间的遗传学特点。方法测定大亚基核糖体DNA基因（LSUrDNA）的ITS区域的碱基序列，以及随机扩增DNA多态性研究。结果12株广东分离株及2株广西分离株的rDNAITS序列测定的结果，通过DDBJ国际GenBank进行菌株同源性搜索与泰国菌株具有10096的同源性。经2个随机引物扩增18株菌，引物ATGS将18株菌分为9个亚型（A1～A9）；引物OPAO15将18株菌分为7个亚型（P1～P7）。12株广东株之间具有较大的遗传差异性，2个引物分别分了4～7个亚型。其中1株分离于广西边界的广东株与广西株具有相同的随机扩增DNA电泳图谱，但另1株远离于广西边界地区的菌株与广西的菌株也具有同样的DNA带型。部分广东株和广西株具有和泰国株同样的遗传相似性。结论12株广东分离的马尔尼菲青霉株与广西和泰国株之间具有一定的遗传差异性，推测广东自然界中极有可能存在多种不同类型的菌株。
The molecular epidemiologic survey was performed in Guangdong province and genetic characteristics of isolates from Guangdong and Guangxi provinces as well as Thailand were compared. The base sequence of ITS region in LUS rDNA was determined by random amplified polymorphic DNA analysis （RAPD）. It was found that 100% homology of the sequences in the ITS region in LUS rDNA existed between 12 isolates from Guangdong province and 2 isolates from Guangxi province as well as with 4 isolates from Thailand by researching for the homology in the DNA Data Bank of Japan （DDBJ）. Nine subtypes were obtained from 18 isolates amplified by primer ATGS （A1-A9）. And 7 subtypes were obtained from 18 isolates amplified by OPAO - 15 primer（Pi- P7）, Some genetic differences were found in 12 isolates from Guangdong province, but one isolate from Guangdong near the city of Guangxi province showed identical DNA pattern with isolates from Guangxi . In addition, some isolates from Guangdong and Guangxi provinces had genetic similarity with isolates from Thailand. It is concluded that 12 isolates of P. marneffei from Guangdong province have genetic similarity with those isolated from Guangxi province and Thailand, suggesting that different types of strains of P. marneffei exist in the natural environment of Guangdong province.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第3期206-208,220,共4页
Chinese Journal of Zoonoses
基金
日本文部省资助项目"FrontierStudiesandInternationalNetworkingofGeneticResourcesinPathogenicFungiandActinomycetes(FN-GRPF)"的一部分。
关键词
马尔尼菲青霉
随机扩增DNA多态性
分子流行病学
Penicillium marneffei
random amplified polymorphic DNA analysis
molecular epidemiology
