摘要
为了观察ＨＢＶ感染时肿瘤坏死因子（ＴＮＦ）对肝细胞（ＰＣ）毒性效应与受体（ＴＮＦＲ）的关系，以进一步阐明慢性乙型肝炎时ＰＣ损伤机制。分别取血清ＨＢＶ感染标志阳性肝癌患者癌周组织及血清ＨＢＶ感染标志阴性肝内胆管结石患者肝组织，作观察组及对照组并行细胞分离培养得ＰＣ及枯否细胞（ＫＣ）。用放射配体结合分析（ＲＢＡ）测定γ－ＩＦＮ外理前后ＰＣＴＮＦＲ数目；将体外活化后ＫＣ与ＰＣ共同培养，测上清液中乳酸脱氢酶（ＬＤＨ）水平。结果显示对照组ＰＣＴＮＦＲ为２９４６±３８１位点／细胞，与观察组相比差异无显著性，ＨＢＶ也不影响受体的上向调节（－ＩＦＮ处理后）；观察组ＰＣ与活化后ＫＣ共同培养２小时，上清中ＬＤＨ显著上升（２．４３３±０．１０５ｕ／ｍｌ），受体增加２～３倍时升高更显著（４．５０２±０．９３１ｕ／ｍ１，ｐ＜０．０１）。因此，ＨＢＶ感染时高表达的ＴＮＦＲ可能增强了ＴＮＦ对ＰＣ毒性效应。
The relationship between cytotoxicity of tumor necrosis factor(TNF)to hepatocytes(PC)and expression of TNF receptors(TNFR)in HBV infection was studied.HBV affected(in-fected or sensitized by HBV)and unaffected (normal)PC were obtained from tumor surrounding tis-sues of patients with hepatocellular carcinoma who showed positive serum marker of HBV and fromlivers of those with intrahapatic bilestone but with negative HBV markers.Radioligand bind assay(RBA)showed that unaffeeted PC TNFR had 2946±38l sites/cell and there was no obvious differ-ence of HBV affected PC TNFR even if they were all upregulated.When HBV affected PC were co-cultured with activated Kupffer cell(KC)for 2 hours,the lactate dehydrogenase(LDH)in super-natant increased obviously(2.443±0.105u/ml)and became much higher(4.502±0.93lu/ml,P<0.0l)while the receptors were 2～3times increased.The increased numbers of TNFR mav have anexaggerated TNF cytotoxicity on PC in HBV-associated PC injury.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1996年第1期20-23,共4页
Chinese Journal of Infectious Diseases
关键词
肿瘤坏死因子
乙型肝炎
受体
放射配体结合
Tumor necrosis factor Viral hepatitis B receptor Radioligan bind assay
