摘要
目的:观察人参茎叶皂苷(ginsenosides,GSS)对热损伤大鼠不同脏器糖皮质激素受体(glucocorticoidreceptor,GR)的影响,并探讨其作用机制。方法:雄性SD大鼠随机分为:(1)正常对照组,室温下饲养,蒸馏水灌胃;(2)GSS治疗组,室温下饲养,GSS灌胃;(3)热损伤模型组,蒸馏水灌胃,制作热损伤模型;(4)热损伤模型GSS治疗组,GSS灌胃,制作热损伤模型。采用放射配体结合法检测大鼠脑、胸腺、肺和肝细胞液GR结合活性;逆转录聚合酶链反应法测定脑、肝细胞液GRmRNA的水平;放射免疫法测定血浆促肾上腺皮质激素(adrenocorticotropin,ACTH)和皮质酮(corticosterone,CS)的浓度。结果:热损伤模型GSS治疗组大鼠脑、肺和肝细胞液GR结合活性以及脑和肝细胞液GRmRNA表达水平均明显高于单纯热损伤模型组(P<0.05或P<0.01);热损伤模型GSS治疗组大鼠血浆ACTH和CS浓度与单纯热损伤模型组比较则无明显差异。结论:GSS可缓解热损伤大鼠不同脏器GR结合活性的下降幅度,其作用机制可能与促进GRmRNA的表达有关。
Objective： To evaluate the effects of ginsenosides （GSS） extracted from ginseng stem and leaves on glucocorticoid receptor （GR） in different viscera in heat-damaged rats, and to find out its action mechanism. Methods： Thirty-two male SD rats were divided into control group and experimental group, and fed 2 mg/d GSS and equal-quantity of distilled water respectively for 7 days. Eight rats of each group were exposed to （42±1）℃ for one hour. The binding activities of GR in brain, thymus, lung and liver cytosols in rats were detected by radioligand binding assay. The expression levels of GR mRNA in brain and liver cytosols were determined by reverse transcription-polymerase chain reaction （RT-PCR） assay. Plasma adreno-corticotropin （ACTH） and corticosterone （CS） concentrations were determined by radioimmunoassay. Results： The binding activities of GR in brain, lung and liver cytosols, and the expression levels of GR mRNA in brain and liver cytosols were all higher in the GSS-treated and heat-damaged rats than those in the untreated heat-damaged rats （P〈0.05 or P〈0.01 ）. There were no significant differences in plasma concentrations of ACTH and CS between the GSS-treated heat-damaged rats and the untreated heat-damaged rats. Conclusion： GSS can lessen the descending degree of the binding activity of GR in brain, thymus, lung and liver cytosols, and such efficacy of GSS may be related to improvment of the expression of GR mRNA.
出处
《中西医结合学报》
CAS
2006年第2期156-159,共4页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金资助项目(No.30271675)
国家自然科学基金杰出青年基金资助项目(No.30025045)
关键词
人参皂苷
人参茎叶
糖皮质激素受体
热损伤
大鼠
ginsenosides
ginseng stem and leaves
glucocorticoid receptor
heat damage
rat
