摘要
AIM： To determine the effect of melatonin pre- and post-treatment on the severity of L-arginine （L-Arg） -induced experimental pancreatitis in rats. METHODS： Male Wistar rats （25） were divided into five groups. Those in group A received two injections of 3.2 g/kg body weight L-Arg i.p. at an interval of 1 h. In group MA, the rats were treated with 50 mg/kg body weight melatonin i.p. 30 min prior to L-Arg administration. In group AM, the rats received the same dose of melatonin 1 h after L-Arg was given. In group M, a single dose of melatonin was administered as described previously. In group C the control animals received physiological saline injections i.p. All rats were exsanguinated 24 h after the second L-Arg injection. RESULTS： L-Arg administration caused severe necrotizing pancreatitis confirmed by the significant elevations in the serum amylase level, the pancreatic weight/body weight ratio （pw/bw）, the pancreatic IL-6 content and the myeloperoxidase activity, relative to the control values. Elevation of the serum amylase level was significantly reduced in rats given melatonin following L-Arg compared to rats injected with L-Arg only. The activities of the pancreatic antioxidant enzymes （Cu/Zn-superoxide dismutase （CulZn-SOD） and catalase （CAT）） were significantly increased 24 h after pancreatitis induction. Melatonin given in advance of L-Arg significantly reduced the pancreatic CAT activity relative to that in the rats treated with L-Arg alone. In the liver, L-Arg significantly increased the lipid peroxidation level, and the glutathione peroxidase and Cu/Zn-SOD activities, whereas the Mn-SOD activity was reduced as compared to the control rats. Melatonin pre-treatment prevented these changes. CONCLUSION： Melatonin is an antioxidant that is able to counteract some of the L-Arg-induced changes during acute pancreatitis, and may therefore be helpful in the supportive therapy of patients with acute necrotizing pancreatitis.
瞄准：决定 melatonin 预告的处理的效果并且在 L 精氨酸(L-Arg ) 的严厉上处理以后在老鼠的导致的试验性的胰腺炎。方法：男 Wistar 老鼠(25 ) 被划分成五个组。那些在 1h 的间隔在组 A 收到了 3.2g/kg 体重 L-Arg i.p 的二注射。在组麻省，老鼠与 50 mg/kg 体重 melatonin i.p 被对待在 L-Arg 管理以前的 30 min。在组 AM，在 L-Arg 被给以后，老鼠收到了 melatonin 1h 的一样的剂量。在组 M， melatonin 的单次量是以前描述了被管理。在组 C，对照动物收到了生理盐水注射 i.p 所有老鼠被给在第二 L-Arg 注射以后的 24 h。结果：引起严重引起坏死胰腺炎的 L-Arg 管理在浆液淀粉酶水平，胰腺的重量 / 身体重量比率(pw/bw ) ，胰腺的 IL-6 内容和 myeloperoxidase 由重要举起证实了活动，相对控制值。浆液淀粉酶水平的举起显著地在与仅仅与 L-Arg 注射的老鼠相比给 melatonin 追随者 L-Arg 的老鼠被减少。胰腺的抗氧化剂酶(Cu/Zn-superoxide 歧化酶(Cu/Zn-SOD ) 和过氧化氢酶(猫)) 的活动显著地被增加在胰腺炎以后的 24 h 感应。显著地在 L-Arg 之前给的 Melatonin 在独自与 L-Arg 对待的老鼠相对那减少了胰腺的猫活动。在肝， L-Arg 显著地每氧化水平，和谷胱甘肽过氧化物酶和 Cu/Zn-SOD 活动增加了类脂化合物，而 Mn 草皮活动作为与控制老鼠相比被减少。Melatonin 预告的处理阻止了这些变化。结论：Melatonin 是能在尖锐胰腺炎期间抵抗一些 L-Arg-induced 变化的一种抗氧化剂，并且可能因此在有尖锐引起坏死胰腺炎的病人的支持的治疗有用。
基金
Supported by the Hungarian Scientific Research Fund (T042589).
