摘要
目的观察第三代二氢吡啶类钙通道阻滞剂拉西地平、氨氯地平对肿瘤坏死因子a诱导的人脐静脉内皮细胞间粘附分子1表达的影响,以探讨拉西地平、氨氯地平抗动脉粥样硬化机制。方法体外培养人脐静脉内皮细胞,以低密度脂蛋白作为载体分别加入不同浓度的拉西地平(5.26×10-5mmol/L、1.58×10-4mmol/L、3.16×10-4mmol/L)和氨氯地平(5.26×10-6mmol/L、1.58×10-5mmol/L、3.16×10-5mmol/L)共同孵育45 min,再加入肿瘤坏死因子a共同孵育6 h,采用流式细胞术和逆转录聚合酶链反应分别测定细胞间粘附分子1蛋白和mRNA的表达。结果不同浓度的拉西地平显著抑制细胞间粘附分子1的表达,随浓度增加,抑制作用逐渐增强;氨氯地平在低浓度时无明显抑制作用,但中、高浓度时可明显抑制细胞间粘附分子1的表达。流式细胞术和逆转录聚合酶链反应的检测结果基本一致。结论拉西地平和氨氯地平均能够显著抑制肿瘤坏死因子a诱导的细胞间粘附分子1表达,但拉西地平的抑制作用强于氨氯地平,可能与其不同的抗氧化活性有关。
Aim To observe the influence of Lacidipine and Amlodipine on tumour necrosis factor （TNF-a） induced expression of intercellular adhesion molecule-1 （ICAM-1 ） in human umbilical vein endothelial cells （hUVEC）. Methods Lacidipine and Amlodipine of different concentrations were incorporated in hUVEC using low density lipoprotein （LDL） as a cartier for 45 minutes respectively. Human recombinant TNF-a was then incubated with hUVEC for 6 hours. The expression of ICAM- 1 in protein level and mRNA level was detected by flow eytometrie technique and RT-PCR. Results Lacidipine, at any concentration, determined a dose-dependent significant decrease of the expression of ICAM-1. Amlodipine determined variable decreases of ICAM-1 at the intermediate and highest concentrations. Conclusion According to the results of flow cytometrie technique and RT-PCR, Lacidipine and Amlodipine exert different effect on the TNF-a induced expression of ICAM-1 in hUVEC, which may be related with their different lipophilicity and antioxidant activity.
出处
《中国动脉硬化杂志》
CAS
CSCD
2005年第5期575-578,共4页
Chinese Journal of Arteriosclerosis
