摘要
为了探讨补肾宁心方对去势雌兔动脉粥样硬化形成的影响及其可能的机制,将26只3月龄新西兰雌兔随机分为正常对照组、假手术组、去势对照组和治疗组(卵巢切除并灌以补肾宁心中药复方),除正常对照组外均于术后2周给予高脂饮食,治疗组同时灌胃给药,连续3个月。12周末测定血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇水平;取主动脉行组织形态学及扫描电镜观察,并应用免疫组织化学方法检测主动脉血管细胞粘附分子1的蛋白表达。结果发现,与去势对照组比较,补肾宁心方对血脂变化无明显影响,但能明显降低主动脉粥样斑块面积与动脉内膜比值,降低内膜中膜厚度比,减轻主动脉病理损害,抑制主动脉血管细胞粘附分子1的蛋白表达。结果提示,补肾宁心方可能通过抑制主动脉血管细胞粘附分子1的表达从而阻止动脉粥样硬化的发生发展。
Aim To study effects of the Bu-shen-ning-xin recipe on atherosclerosis of ovariectomized rabbits and its possible mechanism. Methods Twenty-six female New Zealand white rabbits of three months old were randomly divided into normal group, sham operation group, ovariectomized group and treatment group in which the rabbits were ovariectomized and afforded with Bu-shen-ning-xin decoction. All of rabbits, except normal control, were given high-cholesterol diet two weeks after operation, the treatment group was given additionally by the medication. Twelve weeks later, serum total cholesterol (TC), trig lyceride (TG), high density lipoprotein cholesterol (HDLC) and low density lipoprotein cholesterol (LDLC) levels were measured in routine. The aortas were collected for histomorphometrical evaluation with light and scanning electron microscope. And the expression of vascular cell adhesion molecule-1 (VCAM-1) was analyzed by immunohistochemical techniques. Results Following treatment for 12 weeks, no significant difference was found in serum TC, TG, HDLC and LDLC levels among three groups except the normal. The recipe could lower the area ratios of atherosclerotic lesion to endothelium and the thickness ratios of en-dothelium to the medium in treatment group compared with ovariectomized group. The pathologic damage of aortas were relieved and the expression of VCAM-1 protein in aortas were also significantly reduced following the recipe treatment. Conclusion The Bu-shen-ning-xin recipe could inhibit the VCAM-1 expression of aorta, which contributed to alleviate the pathologic progress of atherosclerosis of the ovariectomized rabbit.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第3期301-304,共4页
Chinese Journal of Arteriosclerosis
基金
上海市科技攻关项目(004019061)资助
