摘要
研究了磷钨酸、十二烷基磺酸钠、单宁酸三种试剂去除液体曲糖化酶中转苷酶的方法。利用正交试验找出了每种方法的最优工艺条件，在最优工艺条件下，磷钨酸法转苷酶去除率为７５．９％，糖化酶活力损失９．６％；十二烷基磺酸钠法转苷酶去除率为６９．１％，糖化酶活力基本不变；单宁酸法转苷酶去除率为７０．３％，糖化酶活力提高９．８％。
This papar deals with the methods for removing glucosyltransferase from submerged mold glucoamylase using tungstophosphoric acid,dodecyl sulphonic acid and tannic acid respectively,The optimal condition is found by normal tests.On the optimal condition,the percentage of removed glucosyltransferase reaches 75.9% and activity of glucoamylase decreases 9.6% when tungstophosphoric acid used.The percentage of removed glucosyltranferase reaches 69.1 and activity of glucoamylase changes very slight by using dodecyl sulphonic acid;the peroentageof removed glucosyltransferase reaches 70.3% and activity of glucoamylase increases 9.8% by means of tannic acid.
出处
《大连轻工业学院学报》
1996年第1期73-78,共6页
Journal of Dalian Institute of Light Industry
关键词
糖化酶
葡萄糖苷转移酶
沉淀
去除
淀粉酶
Glucoamylase
Glucosyitransferase
Tungrtophosphoric acid
Dodapl sulphonic acid
Tannic acid
Submerged mold glucoamylase
