摘要
目的探讨血管生成在脊柱黄韧带骨化中的作用和临床意义。方法3例胸腰椎骨折,10例退变性腰椎管狭窄和14例胸椎黄韧带骨化的后路手术黄韧带标本,分别作为正常组(N)、退变组(D)和骨化组(O),均做HE染色和血管内皮生长因子(VEGF)、环氧化酶2(COX2)和CD34免疫组化染色,测定微血管密度(MVD),BI2000定量分析VEGF、COX2积分光密度值(IOD)。结果正常组纤维母细胞VEGF、COX2表达阴性,退变和骨化组纤维母细胞增生并表达阳性,两组IOD值t检验,骨化组阳性率高于退变组。微血管在正常组稀少,在韧带与骨化移行区增生明显,可见大量CD34阳性表达的血管内皮前体细胞。3组MVD值q检验,骨化和退变组阳性率高于正常组,骨化和退变组无差异。微血管内皮细胞间距增大,管壁缺乏基膜和平滑肌层,管周见阴性染色的细胞聚集。结论脊柱黄韧带纤维母细胞异常分泌VEGF和COX2,促进局部血管新生,是骨化必备条件。
Purpose To discuss the function and clinic significance of angiogensis in spinal ossification of ligamentum flavum. Methods Took ligamentum flavum samples from thoracolumbar vertebral fracture （3 cases）, degenerative lumbar spinal canal stenosis （ 10 cases） and thoracic ossification of ligamentum flavum （14 cases） during spinal posterior operations and classified as normal （N）, degenerative （D） and ossification （O） group respectively. HE staining and VEGF, COX2 and CD34 immunohistochemical staining were performed. Microvessel density （MVD） was counted and VEGF and COX2＇ s integral OD （IOD） were quantified by BI-2000 software. Results Fibroblasts of group N expressed VEGF and COX2 negatively and group D, 0 multiplied and expressed positively. IOD＇s t tests demonstrated there were statistically difference between group 0 and group D. Microvessels were rare in group N and abundant on the transitional zones of ligament-ossification. There existed abundant CD34 ＋ endothelial progenitor cells. MVD＇ s q tests demonstrated group 0 and group D differed from group N, but there were no statistically difference between group 0 and group D. Gaps between endothelia cells enlarged. The basilar membrane and smooth muscle layer were void and CD34-staining pericytes congregated around microvessels. Conclusions The spinal ligamentum flavum fibroblasts excrete abnormally VEGF and COX2. Both promote vasculogenesis locally, which is indispensable to ossification.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2006年第1期69-72,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
脊柱
新生血管
黄韧带骨化
血管内皮生长因子
环氧化酶2
spinal
neovascularization
ossification of ligamentum flavum
vascular endothelial growth factor
cyclooxygenase2
