摘要
目的研究吉西他滨体外对人非小细胞肺癌A549细胞增殖及凋亡的作用。方法应用MTT法,流式细胞仪,Annexin V-PI双标法,Tunel法等多项方法,体外研究吉西他滨对A549细胞的促凋亡作用;采用免疫组化的方法,观察药物作用后bcl-2表达的变化。结果吉西他滨对A549细胞生长具有抑制作用,并有促凋亡效应,对细胞周期的影响表现为S期的阻滞,药物处理的细胞凋亡,可见伴有bcl-2的下调。结论吉西他滨可抑制人非小细胞肺癌A549细胞的生长,并诱导细胞发生凋亡,其诱导凋亡是吉西他滨抗肿瘤作用机制的原因之一,而且凋亡与bcl-2的表达有一定关系。
Objective To observe the effect of proliferation and apoptosis of human non-small cell lung cancer treated by Gemcitabine. Methods The apoptosis of A549 induced by Gemcitabine were detected by Annexin V-PI double-labeling, flow cytometry, TUNEL approach. The changes of the expression of bcl-2 after Gemcitabine treatment was examined by Immunohistochemistry. Results The inhibitory action of cell growth was seen in A549 cell dealing with Gemcitabine. It could also promote the occurrence of apoptosis and induce the S-phase arrest. The apoptosis induced by Gemcitabine was accompanied with the down-regulation of bcl-2. Conclusion Gemcitabine could inhibit the cell proliferation and induce the occurrence of apoptosis of A549 cell. The induced apoptosis, which had certain relationship between the expression of bcl-2, was one of the mechanisms of anti-tumor actions.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第2期100-102,F0003,共4页
Cancer Research on Prevention and Treatment
