摘要
目的:观察银屑病患者外周血单个核细胞(PBMC)中白细胞介素20(IL-20)及其受体(IL-20R)mRNA水平,探讨其与银屑病发生发展的关系。方法:提取外周血总RNA,应用半定量逆转录-聚合酶链反应,以β-actin为内参照,检测IL-20和IL-20R mRNA。结果:银屑病患者PBMC分泌的IL-20水平低于对照组,而IL-20R水平高于对照组。结论:推测IL-20参与银屑病的发病机制,可能是PBMC分泌的IL-20一部分与PBMC自身的受体结合,调节PB-MC自身的免疫功能;另一部分定向转运到皮肤,与其中的受体结合,从而激活Stat信号通路,进一步激活一些与机体炎症反应密切相关的基因,参与银屑病患者机体免疫调节。
Objective： To determine the expression of Interleukin-20 （IL-20） and Interleukin-20 receptor （IL-2OR） in the patients with psoriasis peripheral blood mononuclear cells and controls, RT-PCR assay was used to detect the expression of IL-20 and IL-20 receptor in peripheral blood mononuclear cells （ PB-MC）. Results： It was found that IL-20 mRNA was lower level in PBMC from psoriatic patients than those from controls. But IL-20 receptor mRNA was higher level in samples of patients with psoriasis peripheral blood mononuclear cells than controls. Conclutions： The results suggested that some of IL-20 combind with IL-20 receptor as soon as it was producted; and others were transfered to the target organ skin. IL-20 was expressed in PBMC from both the patients with psoriasis peripheral blood mononuclear cells and controls, but the expression of IL-20 mRNA was down regulated in PBMC from psoriasis peripheral blood mononuclear cells patients.
出处
《江苏大学学报（医学版）》
CAS
2006年第1期44-47,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏省高校自然科学研究指导性计划资助项目(01KJD310007)
