摘要
用重组人γ－干扰素（ＩＦＮ）和肿瘤坏死因子（ＴＮＦ）体外净化骨髓中残存的白血病细胞时，１０００Ｕ／ｍｌＩＦＮ或１００Ｕ／ｍｌＴＮＦ单独应用时仅能清除大约８０％左右，而二者联用时则能起到协同抗白血病的效应，可清除９０％以上残存的白血病细胞，但对ＣＦＵ－ＧＭ的形成却没有协同抑制作用，抑制率仅为５４％。在高热体外净化研究中，单独加热到４１℃作用２ｈ，清除率达９０％，若同时加入１０００Ｕ／ｍｌ的ＩＦＮ，可大大增强高热的净化效果，清除率可达９８％以上，且减轻了高热对ＣＦＵ－ＧＭ的抑制作用，ＣＦＵ－ＧＭ的抑制率下降为２４．５％，与单纯高热净化时的３２．８％相比，Ｐ＜０．０５。
When bone marrow was purged in vitro with recombinant human interferon gamma (rhIFN γ ) 1000 U/ml or tumour necrosis factor(TNF)100 U/ml alone,about 80% of the residual leukemic cells in it could be eliminated.Combination of rhIFN γ and TNF showed a synergic elimination of the residual leukemic cells with a clearance rate of above 90%,but no synergic inhibition of the normal bone marrow CFU GM(inhibitory rate was only 54%)was observed.Hyperthermia(41℃)alone could eliminate more than 90% residual leukemic cells,but when IFN 1000 U/ml was added,the elimination rate was raised to above 98%,while the inhibitory rate of the growth of normal bone marrow progenitor(CFU GM)was decreased to only 24.5% which was much lower than that (32.8%)caused by hyperthermia(41℃),so it is better to raise the temperature for purging in vitro to remove the residual leukemic cells from bone marrow.
出处
《军事医学科学院院刊》
CSCD
北大核心
1996年第1期15-18,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
骨髓移植
体外净化
干扰素
肿瘤坏死因子
interferon,recombinant
leukemia
hyperthermia,induced
bone marrow purging
tumor necrosis factor
