摘要
目的:探讨TNF-α和Fas／FasL的表达及骨髓细胞凋亡与骨髓增生异常综合征(myelodysplastic syndrome,MDS)发生发展的关系。方法:采用 ELISA法检测MDS患者骨髓单个核细胞培养上清液中TNF-α浓度,采用流式细胞术测定骨髓CD 34+细胞Fas、FasL的表达率和细胞凋亡率。结果:各型MDS患者骨髓单个核细胞培养上清液中TNF-α浓度和CD 34+细胞Fas、FasL的表达率均明显高于正常对照组(P< 0．01)。其中RA／RAS组的TNF-α浓度和CD 34+细胞Fas+表达率均明显高于RAEB和RAEB-t组(P<0．001),而RAEB与RAEB-t组组间无明显差异(P值分剐为0．115和0．239);RAEB和RAEB-t组细胞的CD 34+FasL+表迭率明显高于RA／RAS组(P值分别为0．001和0．010)。 RA／RAS和RAEB组的细胞凋亡率均高于对照组(P值均为0．000),而RAEB-t组的细胞凋亡率与对照组相近(P=0．216)。各型MDS患者 TNF-α浓度与CD34+Fas+表达率呈正相关(r=0．570,P=0．012)。而CD 34+细胞凋亡率与TNF-α浓度或CD 34+Fas+表达率之间均无直线相关关系(P值分别为0．103和0．091)。结论:MDS患者骨髓细胞的凋亡受多因素的调节,TNF-α与Fas／FasL系统介导的凋亡以及机体对其调控的失常参与了MDS骨髓无效造血的病理生理过程。
Objective： To investigate the relationship the development and occurrence of myelodysplastic syndrome and TNF-α between the Fas/ FasL expression and apoptosis bone marrow cells. Metbods：The concentration of TNF-α in the supernatant of cultured BMMC were measured by ELISA. The expression of Fas,FasL,apoptosis ratio and bone marrow CD34^＋ cell were evaluated by flow cytometry. Results：The concentration of TNF-α in the supernatant of cultured BMMC and the expression of Fas and FasL of CD34^＋ cell significantly increased in all types of MDS patients compared with control group（P〈0. 01）. The concentration of TNF-α and the expression of CD34^＋ Fas^＋ in RA/RAS patients were much higher as compared with that in RAEB and RAEB-t patients（ P〈 0. 001）, but there was no difference between RAEB and RAEB-t patients （P = 0. 115, 0. 239）. The expression of CD34^＋ FasL^＋ in RAEB and RAE-t patients were higher as compared with that in RA/RAS patients（P=0. 001,0. 010）. Apoptosis of CD34^＋ cell significantly increased in RA/RAS and RAEB patients compared with control group（P=0. 000,0. 000） ,but there was no difference between RAEB-t patients and control group（P= 0. 216）. The concentration of TNF-α was positively correlated with the expression of CD34^＋Fas^＋ in all types of MDS patients（r=0. 570, P=0. 012）, however, apoptosis of CD34^＋ cells and the concentration of TNF-α or the expression of CD34^＋ Fas^＋ have no any statistical correlation（ P= 0. 103,0. 091 ）. Conclusions： apoptosis of bone marrow cells was affected by a lot of factors in MDS,the apoptosis induced by TNF-α and Fas/FasL system can involve in the ineffective hematopoiesis in MDS.
出处
《中国冶金工业医学杂志》
2006年第1期8-10,共3页
Chinese Medical Journal of Metallurgical industry
