摘要
本研究采用RT-PCR法扩增出犬瘟热病毒标准疫苗株的融合蛋白基因(F基因)片段约1499 bp,克隆到pMD18-T载体,酶切鉴定后用一对特异性的引物扩增约732 bp的F蛋白基因片段。并把该基因定向克隆到原核表达载体pET-28a(+)中,转化到宿主菌BL21(DE3)后进行了表达并纯化了该表达产物,Western印迹显示表达产物有良好的抗原性。
RT-PCR was used to amply the fusion （F） gene fragment of Canine Distemper Virus in this study. The product which was about 1499 bp was cloned to pMD18-T vector. Then with a set special primers the F protein gene fragment about 732 bp was amplified. The gene fragment was cloned into pET-28a（＋）vector , and the recombinant plasmid was transformed into E.coli BL21（DE3）. After the recombinant E.coli was induced, the expression protein was puritled. The result of Western-blot showed the recombinant protein had good antigencity.
出处
《上海交通大学学报（农业科学版）》
2006年第1期70-74,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科委攻关项目(No.034909008)
