摘要
为建立适合空间应用的细胞培养技术，利用自行研制的细胞培养反应器，采用微载体悬浮培养系统，对Ｌ９２９细胞和ＣＨＯ细胞进行了培养。结果表明：在面积相同条件下，微载体悬浮培养的细胞产量是不加微载体单层培养的２６倍；微载体浓度为２ｍ／ｍｌ，相对高一些细胞接种密度；１０％左右的血清含量培养基为较优的细胞培养条件。为适合空间条件的需要，采用倍增补液法与微载体球间细胞转移法，探索了细胞扩增培养的途径。同时，对适合空间细胞培养的反应器进行了探索研究。
Two cell lines L929 and CHO were cultured in cell bioreactor with microcarrier suspension culture system. The optimum conditions for cell proliferation were studied.The result showed that microcarrier density 2 mg/ml,an even higher cell moculation density,sernm density abeut 10% were suitable for cells to grow. The cell production under such conditions with microcarrier suspension techniques were 26 times more than that with mono-layer culture. Scale-up of culture volume and beads to beads transfer techniques were devel-oped and new bioreactor were explored to be used in space.
出处
《航天医学与医学工程》
CAS
CSCD
1996年第1期37-41,共5页
Space Medicine & Medical Engineering
关键词
细胞培养
微载体
生物反应器
cell cultures microcarriers cell suspension bioreactors
